Ameloblastin Antibody - #DF8503

(2)   (7)  
製品: Ameloblastin Antibody
カタログ: DF8503
タンパク質の説明: Rabbit polyclonal antibody to Ameloblastin
アプリケーション: WB IHC IF/ICC
Cited expt.: IHC, IF/ICC
反応性: Human, Mouse, Rat
予測: Rabbit
分子量: 48 kDa; 48kD(Calculated).
ユニプロット: Q9NP70
RRID: AB_2841709

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Rabbit(100%)
クローナリティ:
Polyclonal
特異性:
Ameloblastin Antibody detects endogenous levels of total Ameloblastin.
RRID:
AB_2841709
引用形式: Affinity Biosciences Cat# DF8503, RRID:AB_2841709.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AMBN; AMBN_HUMAN; Ameloblastin (enamel matrix protein); Ameloblastin;

免疫原

免疫原:

A synthesized peptide derived from human Ameloblastin, corresponding to a region within the internal amino acids.

Uniprot:

Q9NP70(AMBN_HUMAN) >>Visit HPA database.

遺伝子(ID):
AMBN(258)
発現特異性:
Q9NP70 AMBN_HUMAN:

Ameloblast-specific. Located at the Tomes processes of secretory ameloblasts and in the sheath space between rod-interrod enamel.

タンパク質配列:
MSASKIPLFKMKDLILILCLLEMSFAVPFFPQQSGTPGMASLSLETMRQLGSLQRLNTLSQYSRYGFGKSFNSLWMHGLLPPHSSLPWMRPREHETQQYEYSLPVHPPPLPSQPSLKPQQPGLKPFLQSAAATTNQATALKEALQPPIHLGHLPLQEGELPLVQQQVAPSDKPPKPELPGVDFADPQGPSLPGMDFPDPQGPSLPGLDFADPQGSTIFQIARLISHGPMPQNKQSPLYPGMLYVPFGANQLNAPARLGIMSSEEVAGGREDPMAYGAMFPGFGGMRPGFEGMPHNPAMGGDFTLEFDSPVAATKGPENEEGGAQGSPMPEANPDNLENPAFLTELEPAPHAGLLALPKDDIPGLPRSPSGKMKGLPSVTPAAADPLMTPELADVYRTYDADMTTSVDFQEEATMDTTMAPNSLQTSMPGNKAQEPEMMHDAWHFQEP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
70
Bovine
67
Dog
67
Sheep
58
Xenopus
50
Pig
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Involved in the mineralization and structural organization of enamel.

細胞の位置付け:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ameloblast-specific. Located at the Tomes processes of secretory ameloblasts and in the sheath space between rod-interrod enamel.

タンパク質ファミリー:

Belongs to the ameloblastin family.

参考文献

1). Comprehensive Analysis Identifies Ameloblastin-Related Competitive Endogenous RNA as a Prognostic Biomarker for Testicular Germ Cell Tumour. Cancers, 2022 (PubMed: 35454778) [IF=5.2]

Application: IF/ICC    Species: Rat    Sample: testicular tissue

Figure 1. Low mRNA expression of AMBN in TGCT and its clinical implication. (A) Distribution of AMBN expression in normal testis and TGCT. (B) AMBN expression in rat normal testicular tissue (40×, anti-AMBN (yellow), anti-vimentin (magenta) and DRAQ5 (cyan) to label the nuclei.) (C) Receiver operating characteristic (ROC) curve of AMBN in TGCT (D) AMBN mRNA expression in different TGCT stages (E–G) Kaplan-Meier survival curves were used to compare the difference in progress free interval (PFI) between low and high mRNA expression of AMBN under different conditions (H) OncoPrint plot showing deletion mutations in AMBN gene in TGCT.
2). Revisiting ameloblastin; addressing the EMT-ECM axis above and beyond oral biology. Frontiers in Cell and Developmental Biology, 2023 (PubMed: 38020879)

Application: IHC    Species: Human    Sample:

FIGURE 1 Immunofluorescence imaging of various GI tract tissues. Upper grayscale panels show overview of the tissue in the region where the AMBN signal was detected. Scale bar 200 μm. Lower panels show detection of AMBN immunostaining shown in ImageJ lookup table “red hot” and tissue autofluorescence in cyan. Scale bar 20 μm. GI tract overview diagram was created in Biorender.

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