RASSF2 Antibody - #DF8437
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製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8437, RRID:AB_2841683.
折りたたみ／展開
CENP 34; Centromere protein 34; Ras association (RalGDS/AF 6) domain family 2; Ras association (RalGDS/AF-6) domain family member 2; Ras association domain containing protein 2; Ras association domain-containing protein 2; RASF2_HUMAN; RASFADIN; RASSF2; RP4-599I11.1;
免疫原
Widely expressed with highest levels in brain, placenta, peripheral blood and lung. Frequently down-regulated in lung tumor cell lines.
- P50749 RASF2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDYSHQTSLVPCGQDKYISKNELLLHLKTYNLYYEGQNLQLRHREEEDEFIVEGLLNISWGLRRPIRLQMQDDNERIRPPPSSSSWHSGCNLGAQGTTLKPLTVPKVQISEVDAPPEGDQMPSSTDSRGLKPLQEDTPQLMRTRSDVGVRRRGNVRTPSDQRRIRRHRFSINGHFYNHKTSVFTPAYGSVTNVRINSTMTTPQVLKLLLNKFKIENSAEEFALYVVHTSGEKQKLKATDYPLIARILQGPCEQISKVFLMEKDQVEEVTYDVAQYIKFEMPVLKSFIQKLQEEEDREVKKLMRKYTVLRLMIRQRLEEIAETPATI
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P50749 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S8 | Phosphorylation | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| T137 | Phosphorylation | Uniprot | |
| R142 | Methylation | Uniprot | |
| T143 | Phosphorylation | Uniprot | |
| R144 | Methylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| R151 | Methylation | Uniprot | |
| R165 | Methylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| Y224 | Phosphorylation | Uniprot | |
| Y270 | Phosphorylation | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| Y305 | Phosphorylation | Uniprot | |
| T306 | Phosphorylation | Uniprot | |
| T325 | Phosphorylation | Uniprot |
研究背景
Potential tumor suppressor. Acts as a KRAS-specific effector protein. May promote apoptosis and cell cycle arrest. Stabilizes STK3/MST2 by protecting it from proteasomal degradation.
Phosphorylated by STK3/MST2 and STK4/MST1.
Nucleus. Cytoplasm. Chromosome>Centromere>Kinetochore.
Note: Translocates to the cytoplasm in the presence of STK3/MST2 AND STK4/MST1.
Widely expressed with highest levels in brain, placenta, peripheral blood and lung. Frequently down-regulated in lung tumor cell lines.
Interacts directly with activated KRAS in a GTP-dependent manner. Interacts (via SARAH domain) with STK3/MST2 AND STK4/MST1.
研究領域
· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species. (View pathway)
参考文献
Application: WB Species: human Sample:
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