製品: CD53 Antibody
カタログ: DF8263
タンパク質の説明: Rabbit polyclonal antibody to CD53
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 24 kDa; 24kD(Calculated).
ユニプロット: P19397
RRID: AB_2841552

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製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(90%)
クローナリティ:
Polyclonal
特異性:
CD53 Antibody detects endogenous levels of total CD53.
RRID:
AB_2841552
引用形式: Affinity Biosciences Cat# DF8263, RRID:AB_2841552.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

AI323659; Antigen MOX44 identified by monoclonal antibody MRC-OX44; CD 53; CD53; CD53 antigen; CD53 antigen, isoform CRA_b; CD53 glycoprotein; Cd53 molecule; CD53 tetraspan antigen; CD53_HUMAN; Cell surface glycoprotein CD53; Leukocyte antigen MRC OX-44; Leukocyte surface antigen CD53; MOX 44; MOX44; OTTHUMP00000059505; Ox-44; OX44; RATOX44; Tetraspanin 25; Tetraspanin-25; Tetraspanin25; Transmembrane glycoprotein; TSPAN 25; Tspan-25; TSPAN25;

免疫原

免疫原:

A synthesized peptide derived from human CD53, corresponding to a region within the internal amino acids.

Uniprot:
遺伝子(ID):
発現特異性:
P19397 CD53_HUMAN:

B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells.

タンパク質配列:
MGMSSLKLLKYVLFFFNLLFWICGCCILGFGIYLLIHNNFGVLFHNLPSLTLGNVFVIVGSIIMVVAFLGCMGSIKENKCLLMSFFILLLIILLAEVTLAILLFVYEQKLNEYVAKGLTDSIHRYHSDNSTKAAWDSIQSFLQCCGINGTSDWTSGPPASCPSDRKVEGCYAKARLWFHSNFLYIGIITICVCVIEVLGMSFALTLNCQIDKTSQTIGL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
90
Pig
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Required for efficient formation of myofibers in regenerating muscle at the level of cell fusion. May be involved in growth regulation in hematopoietic cells (By similarity).

細胞の位置付け:

Cell membrane. Cell junction. Membrane>Multi-pass membrane protein.
Note: Concentrates in localized microdomains along the plasma membrane at the contact sites between cells of fused myotubes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells.

タンパク質ファミリー:

Belongs to the tetraspanin (TM4SF) family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.