GBA Antibody - #DF8237
| 製品: | GBA Antibody |
| カタログ: | DF8237 |
| タンパク質の説明: | Rabbit polyclonal antibody to GBA |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse |
| 予測: | Horse, Rabbit, Dog |
| 分子量: | 60 kDa; 60kD(Calculated). |
| ユニプロット: | P04062 |
| RRID: | AB_2841533 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF8237, RRID:AB_2841533.
折りたたみ／展開
Acid beta glucosidase; Acid beta-glucosidase; Alglucerase; Beta glucocerebrosidase; BETA GLUCOSIDASE, ACID; Beta-glucocerebrosidase; betaGC; D glucosyl N acylsphingosine glucohydrolase; D-glucosyl-N-acylsphingosine glucohydrolase; EC 3.2.1.45; GBA; Gba protein; GBA1; GC; GCase; GCB; GLCM_HUMAN; GLUC; Glucocerebrosidase (alt.); Glucocerebrosidase; GLUCOCEREBROSIDASE PSEUDOGENE; Glucosidase beta; Glucosidase, beta, acid; Glucosidase, beta; acid (includes glucosylceramidase); Glucosylceramidase; Imiglucerase; Lysosomal glucocerebrosidase; OTTHUMP00000033992; OTTHUMP00000033993;
免疫原
- P04062 GLCM_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P04062 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N58 | N-Glycosylation | Uniprot | |
| N98 | N-Glycosylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| N185 | N-Glycosylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| K233 | Ubiquitination | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| Y244 | Phosphorylation | Uniprot | |
| N309 | N-Glycosylation | Uniprot | |
| T336 | Phosphorylation | Uniprot | |
| S405 | Phosphorylation | Uniprot | |
| Y412 | Phosphorylation | Uniprot | |
| K512 | Ubiquitination | Uniprot | |
| S523 | Phosphorylation | Uniprot |
研究背景
Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramide/GlcCer into free ceramide and glucose. Thereby, plays a central role in the degradation of complex lipids and the turnover of cellular membranes. Through the production of ceramides, participates to the PKC-activated salvage pathway of ceramide formation. Also plays a role in cholesterol metabolism. May either catalyze the glucosylation of cholesterol, through a transglucosylation reaction that transfers glucose from glucosylceramide to cholesterol. The short chain saturated C8:0-GlcCer and the mono-unsaturated C18:0-GlcCer being the most effective glucose donors for that transglucosylation reaction. Under specific conditions, may alternatively catalyze the reverse reaction, transferring glucose from cholesteryl-beta-D-glucoside to ceramide. Finally, may also hydrolyze cholesteryl-beta-D-glucoside to produce D-glucose and cholesterol.
Lysosome membrane>Peripheral membrane protein>Lumenal side.
Note: Interaction with saposin-C promotes membrane association (PubMed:10781797). Targeting to lysosomes occurs through an alternative MPR-independent mechanism via SCARB2 (PubMed:18022370).
Interacts with saposin-C. Interacts with SCARB2. Interacts with TCP1. May interact with SNCA; may inhibit the glucosylceramidase activity. Interacts with GRN; this interaction prevents aggregation of GBA-SCARB2 complex via interaction with HSPA1A upon stress.
Belongs to the glycosyl hydrolase 30 family.
研究領域
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
· Metabolism > Glycan biosynthesis and metabolism > Other glycan degradation.
· Metabolism > Lipid metabolism > Sphingolipid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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