SOX18 Antibody - #DF8018

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製品: SOX18 Antibody
カタログ: DF8018
タンパク質の説明: Rabbit polyclonal antibody to SOX18
アプリケーション: WB
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Bovine, Dog
分子量: 41 kDa; 41kD(Calculated).
ユニプロット: P35713
RRID: AB_2841390

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Dog(92%)
クローナリティ:
Polyclonal
特異性:
SOX18 Antibody detects endogenous levels of total SOX18.
RRID:
AB_2841390
引用形式: Affinity Biosciences Cat# DF8018, RRID:AB_2841390.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

HLTS; SOX 18; Sox18; SOX18_HUMAN; SRY (sex determining region Y) box 18; SRY box 18; Transcription factor SOX 18; Transcription factor SOX-18;

免疫原

免疫原:

A synthesized peptide derived from human SOX18, corresponding to a region within N-terminal amino acids.

Uniprot:

P35713(SOX18_HUMAN) >>Visit HPA database.

遺伝子(ID):
SOX18(54345)
発現特異性:
P35713 SOX18_HUMAN:

Detected in heart, lung, placenta, skeletal muscle, liver, kidney, spleen, prostate, ovary, msosmall intestine and colon.

タンパク質配列:
MQRSPPGYGAQDDPPARRDCAWAPGHGAAADTRGLAAGPAALAAPAAPASPPSPQRSPPRSPEPGRYGLSPAGRGERQAADESRIRRPMNAFMVWAKDERKRLAQQNPDLHNAVLSKMLGKAWKELNAAEKRPFVEEAERLRVQHLRDHPNYKYRPRRKKQARKARRLEPGLLLPGLAPPQPPPEPFPAASGSARAFRELPPLGAEFDGLGLPTPERSPLDGLEPGEAAFFPPPAAPEDCALRPFRAPYAPTELSRDPGGCYGAPLAEALRTAPPAAPLAGLYYGTLGTPGPYPGPLSPPPEAPPLESAEPLGPAADLWADVDLTEFDQYLNCSRTRPDAPGLPYHVALAKLGPRAMSCPEESSLISALSDASSAVYYSACISG

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Dog
92
Chicken
58
Pig
0
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Transcriptional activator that binds to the consensus sequence 5'-AACAAAG-3' in the promoter of target genes and plays an essential role in embryonic cardiovascular development and lymphangiogenesis. Activates transcription of PROX1 and other genes coding for lymphatic endothelial markers. Plays an essential role in triggering the differentiation of lymph vessels, but is not required for the maintenance of differentiated lymphatic endothelial cells. Plays an important role in postnatal angiogenesis, where it is functionally redundant with SOX17. Interaction with MEF2C enhances transcriptional activation. Besides, required for normal hair development.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in heart, lung, placenta, skeletal muscle, liver, kidney, spleen, prostate, ovary, msosmall intestine and colon.

タンパク質ファミリー:

Binds target DNA via the HMG box domain.

参考文献

1). SOX18 enhances the proliferation and migration of airway smooth muscle cells induced by tumor necrosis factor-α via the regulation of Notch1 signaling. International Immunopharmacology, 2021 (PubMed: 34004439) [IF=4.8]

Application: WB    Species: mouse    Sample: airway smooth muscle cells

Fig. 2. |Knockdown of SOX18 impeded the proliferation and migration of airway smooth muscle cells induced by tumor necrosis factor-α (TNF-α). Airway smooth muscle cells were transfected with scrambled short hairpin RNA (shRNA) or SOX18 shRNA constructs and cultivated for 48 h prior to TNF-α stimulation for another 24 h.(B, C) Levels of the SOX18 protein were determined via western blotting (N = 3).
2). SOX18 exerts tumor-suppressive functions in papillary thyroid carcinoma through inhibition of Wnt/β-catenin signaling. EXPERIMENTAL CELL RESEARCH, 2020 (PubMed: 32858034) [IF=3.3]

Application: WB    Species: human    Sample: papillary thyroid carcinoma

Fig. 1.| Analysis of SOX18 expression in papillary thyroid carcinoma tissues and cell lines.(C, D)Protein expression of SOX18 in clinical specimens of papillary thyroid carcinoma was determined by Western blot. **p < 0.01.

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