APOD Antibody - #DF7987

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製品: APOD Antibody
カタログ: DF7987
タンパク質の説明: Rabbit polyclonal antibody to APOD
アプリケーション: WB IF/ICC
Cited expt.: WB, IF/ICC
反応性: Human, Mouse, Rat
分子量: 30 kDa; 21kD(Calculated).
ユニプロット: P05090
RRID: AB_2841371

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MS Report
HPLC Report
MSDS
説明書
COA
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
APOD Antibody detects endogenous levels of total APOD.
RRID:
AB_2841371
引用形式: Affinity Biosciences Cat# DF7987, RRID:AB_2841371.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

APO D; Apo-D; ApoD; APOD protein; APOD_HUMAN; Apolipoprotein D; ApolipoproteinD;

免疫原

免疫原:

A synthesized peptide derived from human APOD, corresponding to a region within the internal amino acids.

Uniprot:

P05090(APOD_HUMAN) >>Visit HPA database.

遺伝子(ID):
APOD(347)
発現特異性:
P05090 APOD_HUMAN:

Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears.

タンパク質配列:
MVMLLLLLSALAGLFGAAEGQAFHLGKCPNPPVQENFDVNKYLGRWYEIEKIPTTFENGRCIQANYSLMENGKIKVLNQELRADGTVNQIEGEATPVNLTEPAKLEVKFSWFMPSAPYWILATDYENYALVYSCTCIIQLFHVDFAWILARNPNLPPETVDSLKNILTSNNIDVKKMTVTDQVNCPKLS

研究背景

機能:

APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts.

PTMs:

N-glycosylatd. N-glycan heterogeneity at Asn-65: Hex5HexNAc4 (major) and Hex6HexNAc5 (minor); at Asn-98: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (major), dHex1Hex6HexNAc5 (minor) and dHex1Hex7HexNAc6 (minor).

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears.

タンパク質ファミリー:

Belongs to the calycin superfamily. Lipocalin family.

参考文献

1). Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis. Nature Communications, 2022 (PubMed: 35508470) [IF=16.6]

Application: IF/ICC    Species: Mouse    Sample: articular cartilage

Fig. 5 scRNA-seq analysis identified three subsets of chondrocytes. a Medial femoral condyle tissue from the region enclosed by the dotted lines was isolated for cell dissociation. n = 9 mice. Scale bar, 250 μm. b Putative chondrocyte population (171 cells) and unsupervised clustering identified clusters C1, C2, and C3 in cartilage. c Heatmap showing the expression of the top 20 identified significantly differentially expressed genes in clusters C1, C2, and C3. d Immunofluorescence staining of representative markers identified by scRNA-seq in articular cartilage. n = 3 independent experiments. All scale bars, 100 μm. e GO analysis and representative genes for clusters C1, C2, and C3.
2). Identifying the signature of NAD+ metabolism-related genes for immunotherapy of gastric cancer. Heliyon, 2024 (PubMed: 39640811) [IF=4.0]

Application: WB    Species: human    Sample: AGS, SGC-7901 and GES-1 cells

Fig. 12 The expression of Wnt/β-catenin/EMT signaling pathway-related proteins among different cells. (a&c) The expression of Wnt/β-catenin/EMT signaling pathway-related proteins among AGS, SGC-7901 and GES-1 cells. (b&d) The expression of Wnt/β-catenin/EMT signaling pathway-related proteins following knocking down APOD in AGS and SGC-7901 cells. ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001; ∗∗∗∗, P < 0.0001.
3). Proteomics Reveals the Key Molecules Involved in Curcumin-induced Protection Against Sciatic Nerve Injury in Rats. NEUROSCIENCE, 2022 (PubMed: 35870565) [IF=2.9]

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