ORAI1 Antibody - #DF7956

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製品: ORAI1 Antibody
カタログ: DF7956
タンパク質の説明: Rabbit polyclonal antibody to ORAI1
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 50 kDa,60kDa; 33kD(Calculated).
ユニプロット: Q96D31
RRID: AB_2841354

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(100%), Chicken(92%), Xenopus(83%)
クローナリティ:
Polyclonal
特異性:
ORAI1 Antibody detects endogenous levels of total ORAI1.
RRID:
AB_2841354
引用形式: Affinity Biosciences Cat# DF7956, RRID:AB_2841354.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Calcium release activated calcium channel protein 1; Calcium release activated calcium modulator 1; Calcium release-activated calcium channel protein 1; CRACM 1; CRACM1; CRCM1; CRCM1_HUMAN; FLJ14466; Orai 1; ORAI calcium release activated calcium modulator 1; orai1; ORAT1; Protein orai 1; Protein orai-1; TMEM 142A; TMEM142A; Transmembrane protein 142A;

免疫原

免疫原:

A synthesized peptide derived from human ORAI1, corresponding to a region within C-terminal amino acids.

Uniprot:

Q96D31(CRCM1_HUMAN) >>Visit HPA database.

遺伝子(ID):
ORAI1(84876)
タンパク質配列:
MHPEPAPPPSRSSPELPPSGGSTTSGSRRSRRRSGDGEPPGAPPPPPSAVTYPDWIGQSYSEVMSLNEHSMQALSWRKLYLSRAKLKASSRTSALLSGFAMVAMVEVQLDADHDYPPGLLIAFSACTTVLVAVHLFALMISTCILPNIEAVSNVHNLNSVKESPHERMHRHIELAWAFSTVIGTLLFLAEVVLLCWVKFLPLKKQPGQPRPTSKPPASGAAANVSTSGITPGQAAAIASTTIMVPFGLIFIVFAVHFYRSLVSHKTDRQFQELNELAEFARLQDQLDHRGDHPLTPGSHYA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
92
Rabbit
92
Xenopus
83
Zebrafish
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.

PTMs:

N-glycosylated.

Ubiquitinated.

Cys-195 is oxidated, leading to inactivate channel activity.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.
Note: Isoform beta is more mobile in the plasma membrane (PubMed:23307288). Colocalizes with STIM1 at the cell membrane (PubMed:27185316).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the Orai family.

研究領域

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Human Diseases > Immune diseases > Primary immunodeficiency.

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Endocrine system > Renin secretion.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

参考文献

1). Parathyroid Hormone Promotes Human Umbilical Vein Endothelial Cell Migration and Proliferation Through Orai1-Mediated Calcium Signaling. Frontiers in Cardiovascular Medicine, 2022 (PubMed: 35369318) [IF=2.8]

Application: WB    Species: Human    Sample: HUVECs

Figure 1 Effects of parathyroid hormone (PTH) on Orai expression and proliferation of human umbilical vein endothelial cells (HUVECs). (A–D) Representative Western blot images (A) and summary data (B–D) showing Orai1, Orai2, and Orai3 expression level changes in HUVECs after PTH treatment (1, 10, 100 pM) for 24 h compared with the vehicle control (Con). (E) Summary data showing viability of HUVECs treated with PTH (100 pM) for 24 h. (F,G) Representative Western blot images (F) and summary data (G) showing STIM1 expression level changes in HUVECs after PTH treatment (1, 10, 100 pM) for 24 h compared with the vehicle control (Con). Data are shown as the mean ± SEM; n = 3–5. *P < 0.05, **P < 0.01 vs. Con analyzed by one-way analysis of variance followed by Dunnett's multiple comparisons test.

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