FDX1 Antibody - #DF7950

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製品: FDX1 Antibody
カタログ: DF7950
タンパク質の説明: Rabbit polyclonal antibody to FDX1
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 19 kDa; 19kD(Calculated).
ユニプロット: P10109
RRID: AB_2841351

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:200-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
FDX1 Antibody detects endogenous levels of total FDX1.
RRID:
AB_2841351
引用形式: Affinity Biosciences Cat# DF7950, RRID:AB_2841351.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Adrenal ferredoxin; Adrenodoxin; Adrenodoxin, mitochondrial; ADX; ADX_HUMAN; FDX; fdx1; Ferredoxin 1; Ferredoxin-1; Hepatoredoxin; LOH11CR1D; Mitochondrial adrenodoxin; mitochondrial;

免疫原

免疫原:

A synthesized peptide derived from human FDX1, corresponding to a region within the internal amino acids.

Uniprot:

P10109(ADX_HUMAN) >>Visit HPA database.

遺伝子(ID):
FDX1(2230)
発現特異性:
P10109 ADX_HUMAN:

Highest levels in the adrenal gland (at protein level). Also detected in kidney and testis (at protein level).

タンパク質配列:
MAAAGGARLLRAASAVLGGPAGRWLHHAGSRAGSSGLLRNRGPGGSAEASRSLSVSARARSSSEDKITVHFINRDGETLTTKGKVGDSLLDVVVENNLDIDGFGACEGTLACSTCHLIFEDHIYEKLDAITDEENDMLDLAYGLTDRSRLGCQICLTKSMDNMTVRVPETVADARQSIDVGKTS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Essential for the synthesis of various steroid hormones. Participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis. Transfers electrons from adrenodoxin reductase to CYP11A1, a cytochrome P450 that catalyzes cholesterol side-chain cleavage. Does not form a ternary complex with adrenodoxin reductase and CYP11A1 but shuttles between the two enzymes to transfer electrons (By similarity).

細胞の位置付け:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highest levels in the adrenal gland (at protein level). Also detected in kidney and testis (at protein level).

タンパク質ファミリー:

Belongs to the adrenodoxin/putidaredoxin family.

参考文献

1). Photodynamic-Photothermal Switching in Osmium-Ferritin Nanoplatform Activates Near Infrared-Driven Tumor Immunity Reprogramming. ACS Nano, 2025 [IF=16.0]
2). Multifunctional drug delivery nanoparticles for combined chemotherapy/chemodynamic/photothermal therapy against colorectal cancer through synergistic cuproptosis/ferroptosis/apoptosis. Materials today. Bio, 2024 (PubMed: 39850244) [IF=8.7]
3). Eriocitrin inhibits sodium iodate-induced cuproptosis and barrier function impairment in retinal pigment epithelium via SIRT7/YAP/ATP7A pathway. Journal of translational medicine, 2025 (PubMed: 41361469) [IF=7.4]
4). The signature genes of cuproptosis associates with tumor immune microenvironment and predicts prognosis in kidney renal clear cell carcinoma. Frontiers in oncology, 2024 (PubMed: 39206152) [IF=3.5]

Application: WB    Species: human    Sample: Caki-1 cells

Figure 5. Effect of Elesclomol on proliferation and migration of KIRC cells and regulation of FDX1. (A). Caki-1 cells viability was measured using the CCK-8 kit after treating with Elesclomol for 24, 48,72h. (B). EdU staining of Caki-1 cells. Scale bar, 200 μm; n=3; (C). Caki-1 cell EdU flourescence intensity analysis by flow cytometry. (D). The healing/closing state of Caki-1cells by the scratch assay (representative pictures from 3 repeated experiments). (E, F). FDX1 mRNA (E) and protein (F) expression level of Caki-1 cells treated with Elesclomol (500nM) for 48h. (G). The content of Fe-S cluster was tested of Caki-1 cells c treated with Elesclomol (500nM) for 48h. Experiments were repeated three times and the data are expressed as the mean ± SEM. *P < 0.05, ** P
5). Taxifolin regulates SLC31A1-mediated cuproptosis and tumor progression in hepatocellular carcinoma. Human cell, 2025 (PubMed: 39752031) [IF=3.4]

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