F4/80 Antibody - #DF7762

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製品: F4/80 Antibody
カタログ: DF7762
タンパク質の説明: Rabbit polyclonal antibody to F4/80
アプリケーション: WB IF/ICC
Cited expt.:
反応性: Human
予測: Bovine, Sheep
分子量: 102 kDa; 98kD(Calculated).
ユニプロット: Q14246
RRID: AB_2841228

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MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Bovine(83%), Sheep(83%)
クローナリティ:
Polyclonal
特異性:
F4/80 Antibody detects endogenous levels of total F4/80.
RRID:
AB_2841228
引用形式: Affinity Biosciences Cat# DF7762, RRID:AB_2841228.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ADGRE1; Adhesion G protein coupled receptor E1; Adhesion G protein-coupled receptor E1; AGRE1_HUMAN; Cell surface glycoprotein EMR1; Cell surface glycoprotein F4/80; DD7A5 7; Egf like module containing mucin like hormone receptor like 1; Egf like module containing mucin like hormone receptor like sequence 1; EGF like module receptor 1; EGF TM7; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGFTM7; EMR 1; EMR1 hormone receptor; Gpf480; Ly71; Lymphocyte antigen 71; TM7LN3;

免疫原

免疫原:

A synthesized peptide derived from human F4/80, corresponding to a region within C-terminal amino acids.

Uniprot:

Q14246(AGRE1_HUMAN) >>Visit HPA database.

遺伝子(ID):
ADGRE1(2015)
発現特異性:
Q14246 AGRE1_HUMAN:

Expression is restricted to eosinophils.

タンパク質配列:
MRGFNLLLFWGCCVMHSWEGHIRPTRKPNTKGNNCRDSTLCPAYATCTNTVDSYYCACKQGFLSSNGQNHFKDPGVRCKDIDECSQSPQPCGPNSSCKNLSGRYKCSCLDGFSSPTGNDWVPGKPGNFSCTDINECLTSSVCPEHSDCVNSMGSYSCSCQVGFISRNSTCEDVDECADPRACPEHATCNNTVGNYSCFCNPGFESSSGHLSFQGLKASCEDIDECTEMCPINSTCTNTPGSYFCTCHPGFAPSNGQLNFTDQGVECRDIDECRQDPSTCGPNSICTNALGSYSCGCIAGFHPNPEGSQKDGNFSCQRVLFKCKEDVIPDNKQIQQCQEGTAVKPAYVSFCAQINNIFSVLDKVCENKTTVVSLKNTTESFVPVLKQISTWTKFTKEETSSLATVFLESVESMTLASFWKPSANITPAVRTEYLDIESKVINKECSEENVTLDLVAKGDKMKIGCSTIEESESTETTGVAFVSFVGMESVLNERFFKDHQAPLTTSEIKLKMNSRVVGGIMTGEKKDGFSDPIIYTLENIQPKQKFERPICVSWSTDVKGGRWTSFGCVILEASETYTICSCNQMANLAVIMASGELTMDFSLYIISHVGIIISLVCLVLAIATFLLCRSIRNHNTYLHLHLCVCLLLAKTLFLAGIHKTDNKMGCAIIAGFLHYLFLACFFWMLVEAVILFLMVRNLKVVNYFSSRNIKMLHICAFGYGLPMLVVVISASVQPQGYGMHNRCWLNTETGFIWSFLGPVCTVIVINSLLLTWTLWILRQRLSSVNAEVSTLKDTRLLTFKAFAQLFILGCSWVLGIFQIGPVAGVMAYLFTIINSLQGAFIFLIHCLLNGQVREEYKRWITGKTKPSSQSQTSRILLSSMPSASKTG

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
83
Sheep
83
Dog
67
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expression is restricted to eosinophils.

タンパク質ファミリー:

Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.

参考文献

1). Huanglian Jiedu Decoction ameliorates DSS-induced colitis in mice via the JAK2/STAT3 signalling pathway. Chinese Medicine, 2020 (PubMed: 32411291) [IF=5.3]
2). Paracrine effect of the stromal vascular fraction containing M2 macrophages on human chondrocytes through the Smad2/3 signaling pathway. Journal of Cellular Physiology, 2022 (PubMed: 35766589) [IF=4.5]
3). Pioglitazone decreased renal calcium oxalate crystal formation by suppressing M1 macrophage polarization via the PPAR-γ-miR-23 axis. AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2019 (PubMed: 31091119) [IF=3.7]

Application: IHC    Species: mouse    Sample: kidneys

Fig. 2.| Evaluation of expression of peroxisome proliferator-activated receptor (PPAR)-,macrophage (Mp)-related molecules, proinflammatory cytokines, and anti-inflammatory cytokines. A: immunohistochemical distribution of the expression of PPAR-, proinflammatory cytokines, and Mp-related molecules in the kidneys as well as the ratio of areas with positive expression. PPAR- expression in pioglitazone (PGZ)-treated groups was significantly higher than in other groups. Expression of monocyte chemotactic protein (MCP)-1, F4/80, inducible nitric oxide synthase (iNOS), arginine 1 (ARG1), interferon regulatory factor 1 (IRF1), and PKNOX1 was significantly higher in 3-day (d) glyoxylic acid (GA)- and 6-day GA-treated groups than in the control group and was significantly lower in PGZ-treated groups than in the 6-day GA group. Original magnification: 200.
4). Rosiglitazone Suppresses Renal Crystal Deposition by Ameliorating Tubular Injury Resulted from Oxidative Stress and Inflammatory Response via Promoting the Nrf2/HO-1 Pathway and Shifting Macrophage Polarization. Oxidative Medicine and Cellular Longevity, 2021 (PubMed: 34691355)

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