Bone Sialoprotein Antibody - #DF7738

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製品: Bone Sialoprotein Antibody
カタログ: DF7738
タンパク質の説明: Rabbit polyclonal antibody to Bone Sialoprotein
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
分子量: 35 kDa.; 35kD(Calculated).
ユニプロット: P21815
RRID: AB_2841206

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Bone Sialoprotein Antibody detects endogenous levels of total Bone Sialoprotein.
RRID:
AB_2841206
引用形式: Affinity Biosciences Cat# DF7738, RRID:AB_2841206.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

BNSP; Bone sialoprotein 2; Bone sialoprotein II; BSP; BSP II; BSPII; Cell binding sialoprotein; Cell-binding sialoprotein; IBSP; Integrin binding sialoprotein; Integrin-binding sialoprotein; SIAL_HUMAN; SPII;

免疫原

免疫原:
Uniprot:

P21815(SIAL_HUMAN) >>Visit HPA database.

遺伝子(ID):
IBSP(3381)
タンパク質配列:
MKTALILLSILGMACAFSMKNLHRRVKIEDSEENGVFKYRPRYYLYKHAYFYPHLKRFPVQGSSDSSEENGDDSSEEEEEEEETSNEGENNEESNEDEDSEAENTTLSATTLGYGEDATPGTGYTGLAAIQLPKKAGDITNKATKEKESDEEEEEEEEGNENEESEAEVDENEQGINGTSTNSTEAENGNGSSGGDNGEEGEEESVTGANAEDTTETGRQGKGTSKTTTSPNGGFEPTTPPQVYRTTSPPFGKTTTVEYEGEYEYTGANEYDNGYEIYESENGEPRGDNYRAYEDEYSYFKGQGYDGYDGQNYYHHQ

PTMs - P21815 基板として

Site PTM Type Enzyme
T3 Phosphorylation
S9 Phosphorylation
S18 Phosphorylation
S31 Phosphorylation
Y39 Phosphorylation
T119 O-Glycosylation
T122 O-Glycosylation
T207 Phosphorylation
S225 Phosphorylation
T227 O-Glycosylation
T228 O-Glycosylation
T228 Phosphorylation
T229 O-Glycosylation
T238 O-Glycosylation
T239 O-Glycosylation
T246 Phosphorylation

研究背景

機能:

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.

PTMs:

N-glycosylated; glycans consist of sialylated and core-fucosylated bi-, tri- and tetraantennary chains.

O-glycosylated at eight sites; mucin-type glycans contain Gal, GlcNAc, GalNAc and terminal NeuAc.

Sulfated on either Tyr-313 or Tyr-314.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

研究領域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

参考文献

1). Osteogenic potential evaluation of biotin combined with magnesium-doped hydroxyapatite sustained-release film. Materials science & engineering-C, Materials for biological applications (PubMed: 35581076) [IF=7.9]
2). Metformin protects against vascular calcification through the selective degradation of Runx2 by the p62 autophagy receptor. Journal of Cellular Physiology (PubMed: 36166694) [IF=5.6]

Application: WB    Species: Mouse    Sample: VSMCs

FIGURE 1Metformin activates AMPK and calcification inVSMCs. VSMCs were cultured with high phosphate (3 mM Pi) and metformin (met)for 7 days. (a) Calcium content (mg/mg protein) of cells treated with metformin (0.5−1.5 mM), (n= 3). (b) Representative alizarin red images ofcells treated with metformin (1 mM). (c) Representative immunoblots (d) immunoblot quantification showing the effect of metformin (1 mM) onexpression of osteogenic markers osterix, osteocalcin, and bone sialoprotein compared withβ‐actin (n= 3). (e) Representative immunoblots and(f) immunoblot quantification showing the effect of metformin (1 mM) on the phosphorylation of AMPK compared with total AMPK (n= 4).AMPK, adenosine monophosphate‐activated protein kinase; VSMCs, vascular smooth muscle cells.PHADWALET AL.|4307
3). Metformin ameliorates valve interstitial cell calcification by promoting autophagic flux. Scientific reports (PubMed: 38052777) [IF=4.6]
4). Static magnetic field promotes proliferation, migration, differentiation, and AKT activation of periodontal ligament stem cells. Cells Tissues Organs (PubMed: 35344952) [IF=2.7]

Application: WB    Species: Human    Sample: PDLSCs

Fig. 4. SMF promoted the osteogenic differentiation of PDLSCs. a mRNA expression of ALP, BSP, and RUNX2was detected by qRT–PCR after cells were cultured for 7 days. b, c The protein expression levels of ALP, BSP, and RUNX2 were detected by Western blot after cells were cultured for 7 and 14 days. All experiments were performed in triplicate, and the values are expressed as the mean ± SD. *p< 0.05, **p< 0.01.
5). SDF-1 induces directional chemotaxis of BMSCs at the intervertebral fusion site and promotes osteogenic differentiation by regulating Wnt/β-catenin in the bone marrow chimera spinal intervertebral fusion mouse model.. Turkish journal of biology = Turk biyoloji dergisi (PubMed: 37529111) [IF=2.2]

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