製品: POFUT2 Antibody
カタログ: AF0588
タンパク質の説明: Rabbit polyclonal antibody to POFUT2
アプリケーション: WB IHC
反応性: Human, Mouse
予測: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog
分子量: 49kDa; 50kD(Calculated).
ユニプロット: Q9Y2G5
RRID: AB_2834400

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製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Zebrafish(88%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(88%)
クローナリティ:
Polyclonal
特異性:
POFUT2 Antibody detects endogenous levels of total POFUT2.
RRID:
AB_2834400
引用形式: Affinity Biosciences Cat# AF0588, RRID:AB_2834400.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

C21orf80; EC 2.4.1.221; FUT 13; FUT13; GDP fucose protein O fucosyltransferase 2; GDP-fucose protein O-fucosyltransferase 2; KIAA0958; O FucT 2; O-FucT-2; OFucT2; OFUT2_HUMAN; Peptide O fucosyltransferase 2; Peptide-O-fucosyltransferase 2; POFUT 2; POFUT2; Protein O fucosyltransferase 2;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q9Y2G5 OFUT2_HUMAN:

Isoform A is expressed in fetal liver and peripheral blood lymphocytes. Isoform B is expressed in spleen, lung, testis, bone marrow, thymus, pancreas, prostate, fetal brain, fetal liver and fetal kidney. Isoform C is expressed in brain, heart, spleen, liver, lung, stomach, testis, placenta, skin, thymus, pancreas, mammary gland, prostate, fetal brain, fetal liver and fetal heart.

タンパク質の説明:
POFUT2 Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in thrombospondin type 1 repeats. Belongs to the glycosyltransferase 68 family. 3 isoforms of the human protein are produced by alternative splicing.
タンパク質配列:
MATLSFVFLLLGAVSWPPASASGQEFWPGQSAADILSGAASRRRYLLYDVNPPEGFNLRRDVYIRIASLLKTLLKTEEWVLVLPPWGRLYHWQSPDIHQVRIPWSEFFDLPSLNKNIPVIEYEQFIAESGGPFIDQVYVLQSYAEGWKEGTWEEKVDERPCIDQLLYSQDKHEYYRGWFWGYEETRGLNVSCLSVQGSASIVAPLLLRNTSARSVMLDRAENLLHDHYGGKEYWDTRRSMVFARHLREVGDEFRSRHLNSTDDADRIPFQEDWMKMKVKLGSALGGPYLGVHLRRKDFIWGHRQDVPSLEGAVRKIRSLMKTHRLDKVFVATDAVRKEYEELKKLLPEMVRFEPTWEELELYKDGGVAIIDQWICAHARFFIGTSVSTFSFRIHEEREILGLDPKTTYNRFCGDQEKACEQPTHWKITY

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Rabbit
100
Dog
88
Zebrafish
88
Chicken
75
Bovine
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y2G5 基板として

Site PTM Type Enzyme
Y182 Phosphorylation
T185 Phosphorylation
N189 N-Glycosylation
S191 Phosphorylation
S194 Phosphorylation
N259 N-Glycosylation

研究背景

機能:

Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X(2,3)-S/T-C2-X(2)-G of thrombospondin type 1 repeats where C1 and C2 are the first and second cysteines, respectively. O-fucosylates members of several protein families including the ADAMTS family, the thrombosporin (TSP) and spondin families. The O-fucosylation of TSRs is also required for restricting epithelial to mesenchymal transition (EMT), maintaining the correct patterning of mesoderm and localization of the definite endoderm (By similarity). Required for the proper secretion of ADAMTS family members such as ADAMSL1 and ADAMST13.

細胞の位置付け:

Endoplasmic reticulum. Golgi apparatus.
Note: Mainly located in the endoplasmic reticulum.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Isoform A is expressed in fetal liver and peripheral blood lymphocytes. Isoform B is expressed in spleen, lung, testis, bone marrow, thymus, pancreas, prostate, fetal brain, fetal liver and fetal kidney. Isoform C is expressed in brain, heart, spleen, liver, lung, stomach, testis, placenta, skin, thymus, pancreas, mammary gland, prostate, fetal brain, fetal liver and fetal heart.

タンパク質ファミリー:

Belongs to the glycosyltransferase 68 family.

研究領域

· Metabolism > Glycan biosynthesis and metabolism > Other types of O-glycan biosynthesis.

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