| 製品: | PLEC Antibody |
| カタログ: | DF7631 |
| タンパク質の説明: | Rabbit polyclonal antibody to PLEC |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Bovine, Horse |
| 分子量: | 531 KD(Observed); 532kD(Calculated). |
| ユニプロット: | Q15149 |
| RRID: | AB_2841116 |
Control Products
製品説明
ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Horse(100%)
クローナリティ:
Polyclonal
特異性:
PLEC Antibody detects endogenous levels of total PLEC.
RRID:
AB_2841116
引用形式: Affinity Biosciences Cat# DF7631, RRID:AB_2841116.
引用形式: Affinity Biosciences Cat# DF7631, RRID:AB_2841116.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
EBS1; EBSO; HD1; Hemidesmosomal protein 1; PCN; pleC; PLEC_HUMAN; PLEC1; PLEC1b; Plectin 1; Plectin 1 intermediate filament binding protein 500kDa; Plectin 6; Plectin; Plectin-1; PLTN;
免疫原
免疫原:
A synthesized peptide derived from human PLEC, corresponding to a region within N-terminal amino acids.
Uniprot:
遺伝子(ID):
発現特異性:
タンパク質配列:
- Q15149 PLEC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
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種類予測
種類予測:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Horse
100
Bovine
100
Pig
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
研究背景
機能:
Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofiber integrity.
PTMs:
Phosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis.
細胞の位置付け:
Cytoplasm>Cytoskeleton. Cell junction>Hemidesmosome.
組織特異性:
Widely expressed with highest levels in muscle, heart, placenta and spinal cord.
タンパク質ファミリー:
The N-terminus interacts with actin, the C-terminus with vimentin, desmin, GFAP, cytokeratins, lamin B; whereas both the N- and the C-terminus can bind integrin beta-4.
Belongs to the plakin or cytolinker family.
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