製品: CKII alpha Antibody
カタログ: DF7563
タンパク質の説明: Rabbit polyclonal antibody to CKII alpha
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 45 kDa; 45kD(Calculated).
ユニプロット: P68400
RRID: AB_2841057

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(92%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
クローナリティ:
Polyclonal
特異性:
CKII alpha Antibody detects endogenous levels of total CKII alpha.
RRID:
AB_2841057
引用形式: Affinity Biosciences Cat# DF7563, RRID:AB_2841057.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Casein kinase 2 alpha 1 polypeptide; Casein kinase II alpha 1; Casein kinase II alpha 1 subunit; Casein kinase II alpha subunit; Casein kinase II subunit alpha; CK II alpha; CK II; CK2 alpha; CK2 catalytic subunit alpha; CK2A1; CKII; CKIIalpha; CSK21_HUMAN; CSNK2A1;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P68400 CSK21_HUMAN:

Expressed in gastric carcinoma tissue and the expression gradually increases with the progression of the carcinoma (at protein level).

タンパク質配列:
MSGPVPSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKRWERFVHSENQHLVSPEALDFLDKLLRYDHQSRLTAREAMEHPYFYTVVKDQARMGSSSMPGGSTPVSSANMMSGISSVPTPSPLGPLAGSPVIAAANPLGMPVPAAAGAQQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
92
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P68400 基板として

Site PTM Type Enzyme
S2 Phosphorylation
S7 Phosphorylation
Y12 Phosphorylation
T13 Phosphorylation P31749 (AKT1)
T17 Phosphorylation
K49 Acetylation
K49 Ubiquitination
Y50 Phosphorylation
S51 Phosphorylation
T60 Phosphorylation
K68 Ubiquitination
K71 Ubiquitination
K83 Ubiquitination
R89 Methylation
K102 Acetylation
K102 Ubiquitination
K122 Acetylation
K122 Ubiquitination
T127 Phosphorylation
T129 Phosphorylation
Y131 Phosphorylation
K158 Ubiquitination
Y182 Phosphorylation P68400 (CSNK2A1)
S194 Phosphorylation
K229 Ubiquitination
Y239 Phosphorylation
K247 Ubiquitination
Y255 Phosphorylation P07948 (LYN) , P09769 (FGR)
Y257 Phosphorylation
K260 Ubiquitination
S277 Phosphorylation
S287 Phosphorylation
K303 Ubiquitination
Y323 Phosphorylation
K329 Acetylation
K329 Ubiquitination
T344 Phosphorylation P06493 (CDK1)
T360 Phosphorylation P28482 (MAPK1) , P06493 (CDK1)
S362 Phosphorylation P06493 (CDK1) , P28482 (MAPK1)
S370 Phosphorylation P06493 (CDK1)

PTMs - P68400 酵素として

Substrate Site Source
O00170 (AIP) S43 Uniprot
O14737 (PDCD5) S119 Uniprot
O14950 (MYL12B) T135 Uniprot
O14958 (CASQ2) S385 Uniprot
O14958 (CASQ2) S393 Uniprot
O15259 (NPHP1) S121 Uniprot
O15259 (NPHP1) S123 Uniprot
O15259 (NPHP1) S126 Uniprot
O15266 (SHOX) S106 Uniprot
O15379 (HDAC3) S424 Uniprot
O15392 (BIRC5) T48 Uniprot
O43156 (TTI1) S828 Uniprot
O43395 (PRPF3) T494 Uniprot
O43852-15 (CALU) T73 Uniprot
O43896 (KIF1C) S1092 Uniprot
O60341 (KDM1A) S131 Uniprot
O60341 (KDM1A) S137 Uniprot
O60671 (RAD1) S280 Uniprot
O60671 (RAD1) S282 Uniprot
O60936 (NOL3) T149 Uniprot
O75376 (NCOR1) S2436 Uniprot
O75379-1 (VAMP4) S30 Uniprot
O75496 (GMNN) S184 Uniprot
O75582 (RPS6KA5) S757 Uniprot
O75582 (RPS6KA5) S758 Uniprot
O75582 (RPS6KA5) S759 Uniprot
O75582 (RPS6KA5) S760 Uniprot
O75582 (RPS6KA5) T793 Uniprot
O75676 (RPS6KA4) S324 Uniprot
O75822 (EIF3J) S127 Uniprot
O75925 (PIAS1) S466 Uniprot
O75925 (PIAS1) S467 Uniprot
O75925 (PIAS1) S468 Uniprot
O76061 (STC2) S288 Uniprot
O95477 (ABCA1) T1242 Uniprot
O95477 (ABCA1) T1243 Uniprot
O95477 (ABCA1) S1255 Uniprot
O95786 (DDX58) T770 Uniprot
O95786 (DDX58) S854 Uniprot
O95786 (DDX58) S855 Uniprot
O95863 (SNAI1) S11 Uniprot
O95863 (SNAI1) S92 Uniprot
O95905 (ECD) S503 Uniprot
O95905 (ECD) S505 Uniprot
O95905 (ECD) S518 Uniprot
P00736 (C1R) S206 Uniprot
P01106-2 (MYC) T73 Uniprot
P01106 (MYC) S249 Uniprot
P01106 (MYC) S250 Uniprot
P01106 (MYC) S252 Uniprot
P01106 (MYC) S347 Uniprot
P01106 (MYC) S348 Uniprot
P02649 (APOE) S314 Uniprot
P02671 (FGA) S542 Uniprot
P02671 (FGA) S609 Uniprot
P03372 (ESR1) S167 Uniprot
P03372 (ESR1) S282 Uniprot
P03372 (ESR1) S559 Uniprot
P04004 (VTN) T69 Uniprot
P04004 (VTN) T76 Uniprot
P04198 (MYCN) S261 Uniprot
P04198 (MYCN) S263 Uniprot
P04637-1 (TP53) T18 Uniprot
P04637 (TP53) S149 Uniprot
P04637 (TP53) T150 Uniprot
P04637 (TP53) T155 Uniprot
P04637 (TP53) S392 Uniprot
P04818 (TYMS) S124 Uniprot
P05114 (HMGN1) S7 Uniprot
P05114 (HMGN1) S8 Uniprot
P05114 (HMGN1) S86 Uniprot
P05114 (HMGN1) S89 Uniprot
P05114 (HMGN1) S99 Uniprot
P05412 (JUN) T231 Uniprot
P05412 (JUN) S243 Uniprot
P05412 (JUN) S249 Uniprot
P05455 (SSB) S366 Uniprot
P05549 (TFAP2A) S429 Uniprot
P06127 (CD5) S482 Uniprot
P06127 (CD5) S483 Uniprot
P06127 (CD5) S485 Uniprot
P06401-1 (PGR) S81 Uniprot
P06493 (CDK1) S39 Uniprot
P06744 (GPI) S185 Uniprot
P07900 (HSP90AA1) T36 Uniprot
P07900-1 (HSP90AA1) S231 Uniprot
P07900-1 (HSP90AA1) S263 Uniprot
P07900-2 (HSP90AA1) S353 Uniprot
P07900-2 (HSP90AA1) S385 Uniprot
P07910-2 (HNRNPC) S247 Uniprot
P07910-1 (HNRNPC) S260 Uniprot
P08047-1 (SP1) T579 Uniprot
P08195 (SLC3A2) S406 Uniprot
P08195 (SLC3A2) S408 Uniprot
P08195 (SLC3A2) S410 Uniprot
P08195 (SLC3A2) S527 Uniprot
P08195 (SLC3A2) S531 Uniprot
P08238 (HSP90AB1) S226 Uniprot
P08238 (HSP90AB1) S255 Uniprot
P08473 (MME) S6 Uniprot
P08833 (IGFBP1) S126 Uniprot
P08833 (IGFBP1) S144 Uniprot
P08833 (IGFBP1) S194 Uniprot
P09497-2 (CLTB) S11 Uniprot
P09497-1 (CLTB) S13 Uniprot
P09629 (HOXB7) S132 Uniprot
P09629 (HOXB7) S133 Uniprot
P09629 (HOXB7) T203 Uniprot
P09629 (HOXB7) T204 Uniprot
P09651 (HNRNPA1) S199 Uniprot
P09958 (FURIN) S773 Uniprot
P09958 (FURIN) S775 Uniprot
P10242-1 (MYB) S11 Uniprot
P10242-1 (MYB) S12 Uniprot
P10243 (MYBL1) S7 Uniprot
P10244 (MYBL2) T18 Uniprot
P10451-5 (SPP1) S148 Uniprot
P10646 (TFPI) S30 Uniprot
P11387 (TOP1) S10 Uniprot
P11387 (TOP1) S506 Uniprot
P11388-1 (TOP2A) T1343 Uniprot
P11388 (TOP2A) S1365 Uniprot
P11388 (TOP2A) S1377 Uniprot
P11388 (TOP2A) S1469 Uniprot
P11388 (TOP2A) S1525 Uniprot
P11473-1 (VDR) S208 Uniprot
P11717 (IGF2R) S2409 Uniprot
P11717 (IGF2R) S2484 Uniprot
P11831 (SRF) S77 Uniprot
P11831 (SRF) S79 Uniprot
P11831 (SRF) S83 Uniprot
P11831 (SRF) S85 Uniprot
P11836-1 (MS4A1) S231 Uniprot
P11836-1 (MS4A1) T250 Uniprot
P11836-1 (MS4A1) S289 Uniprot
P12259 (F5) S692 Uniprot
P12259 (F5) S720 Uniprot
P12821 (ACE) S1299 Uniprot
P12830-1 (CDH1) S838 Uniprot
P12830-1 (CDH1) S847 Uniprot
P12830 (CDH1) S850 Uniprot
P12830-1 (CDH1) S851 Uniprot
P12830 (CDH1) S853 Uniprot
P13349 (MYF5) S49 Uniprot
P13349 (MYF5) S133 Uniprot
P13569 (CFTR) S422 Uniprot
P13569 (CFTR) S511 Uniprot
P13569 (CFTR) T1471 Uniprot
P13807-1 (GYS1) S10 Uniprot
P13807-1 (GYS1) S647 Uniprot
P13807-1 (GYS1) S652 Uniprot
P13807-1 (GYS1) S653 Uniprot
P13807-1 (GYS1) S698 Uniprot
P13807-1 (GYS1) S701 Uniprot
P13807-1 (GYS1) T713 Uniprot
P14317 (HCLS1) T16 Uniprot
P14317 (HCLS1) T23 Uniprot
P14598 (NCF1) S208 Uniprot
P14598 (NCF1) S283 Uniprot
P14598 (NCF1) S348 Uniprot
P16220 (CREB1) S108 Uniprot
P16220 (CREB1) S111 Uniprot
P16949-1 (STMN1) S16 Uniprot
P16949-1 (STMN1) S63 Uniprot
P17096-2 (HMGA1) S88 Uniprot
P17096-2 (HMGA1) S91 Uniprot
P17096-2 (HMGA1) S92 Uniprot
P17096-1 (HMGA1) S99 Uniprot
P17096-1 (HMGA1) S102 Uniprot
P17096-1 (HMGA1) S103 Uniprot
P17677-1 (GAP43) S202 Uniprot
P17677-1 (GAP43) S203 Uniprot
P17931 (LGALS3) S6 Uniprot
P17931 (LGALS3) S12 Uniprot
P17936 (IGFBP3) S138 Uniprot
P17936 (IGFBP3) S140 Uniprot
P17936-2 (IGFBP3) S144 Uniprot
P17936-2 (IGFBP3) S146 Uniprot
P17936 (IGFBP3) S194 Uniprot
P18031 (PTPN1) S352 Uniprot
P18031 (PTPN1) S378 Uniprot
P18031 (PTPN1) S386 Uniprot
P18146 (EGR1) S378 Uniprot
P18146 (EGR1) T391 Uniprot
P18146 (EGR1) T526 Uniprot
P18846 (ATF1) S36 Uniprot
P18846 (ATF1) S38 Uniprot
P18846 (ATF1) S41 Uniprot
P18846 (ATF1) S44 Uniprot
P18846 (ATF1) S47 Uniprot
P18846 (ATF1) S50 Uniprot
P18846 (ATF1) S51 Uniprot
P18846 (ATF1) S63 Uniprot
P18858-1 (LIG1) S66 Uniprot
P18887 (XRCC1) S408 Uniprot
P18887 (XRCC1) S409 Uniprot
P18887 (XRCC1) S410 Uniprot
P18887 (XRCC1) S416 Uniprot
P18887 (XRCC1) S418 Uniprot
P18887 (XRCC1) S421 Uniprot
P18887 (XRCC1) S461 Uniprot
P18887 (XRCC1) S475 Uniprot
P18887 (XRCC1) S485 Uniprot
P18887 (XRCC1) T488 Uniprot
P18887 (XRCC1) Y515 Uniprot
P18887 (XRCC1) S518 Uniprot
P18887 (XRCC1) T519 Uniprot
P18887 (XRCC1) T523 Uniprot
P18887 (XRCC1) S525 Uniprot
P18887 (XRCC1) S578 Uniprot
P19447 (ERCC3) S751 Uniprot
P20042 (EIF2S2) S2 Uniprot
P20042 (EIF2S2) S67 Uniprot
P21673 (SAT1) T10 Uniprot
P21673 (SAT1) S146 Uniprot
P21673 (SAT1) S149 Uniprot
P22736 (NR4A1) S152 Uniprot
P23760 (PAX3) S205 Uniprot
P24046-1 (GABRR1) S402 Uniprot
P24534 (EEF1B2) S106 Uniprot
P24534 (EEF1B2) S112 Uniprot
P25490 (YY1) S118 Uniprot
P25788-1 (PSMA3) S243 Uniprot
P25963 (NFKBIA) S32 Uniprot
P25963 (NFKBIA) S36 Uniprot
P25963 (NFKBIA) S283 Uniprot
P25963 (NFKBIA) S288 Uniprot
P25963 (NFKBIA) T291 Uniprot
P25963 (NFKBIA) S293 Uniprot
P25963 (NFKBIA) T299 Uniprot
P27540-1 (ARNT) S77 Uniprot
P27695 (APEX1) S123 Uniprot
P27986 (PIK3R1) S608 Uniprot
P28482 (MAPK1) S246 Uniprot
P28482 (MAPK1) S248 Uniprot
P29590 (PML) S518 Uniprot
P29590 (PML) S560 Uniprot
P29590 (PML) S561 Uniprot
P29590 (PML) S562 Uniprot
P29590 (PML) S565 Uniprot
P29692 (EEF1D) S162 Uniprot
P30203 (CD6) S480 Uniprot
P30203 (CD6) S482 Uniprot
P30203 (CD6) S484 Uniprot
P30260 (CDC27) S154 Uniprot
P30291 (WEE1) S121 Uniprot
P30305-2 (CDC25B) S172 Uniprot
P30305-2 (CDC25B) S173 Calculation

研究背景

機能:

Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV. Phosphorylates PML at 'Ser-565' and primes it for ubiquitin-mediated degradation. Plays an important role in the circadian clock function by phosphorylating ARNTL/BMAL1 at 'Ser-90' which is pivotal for its interaction with CLOCK and which controls CLOCK nuclear entry. Phosphorylates CCAR2 at 'Thr-454' in gastric carcinoma tissue.

PTMs:

Phosphorylated at Thr-344, Thr-360, Ser-362 and Ser-370 by CDK1 in prophase and metaphase and dephosphorylated during anaphase. Phosphorylation does not directly affect casein kinase 2 activity, but may contribute to its regulation by forming binding sites for interacting proteins and/or targeting it to different compartments.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in gastric carcinoma tissue and the expression gradually increases with the progression of the carcinoma (at protein level).

サブユニット構造:

Heterotetramer composed of two catalytic subunits (alpha chain and/or alpha' chain) and two regulatory subunits (beta chains). The tetramer can exist as a combination of 2 alpha/2 beta, 2 alpha'/2 beta or 1 alpha/1 alpha'/2 beta subunits. Also part of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, which forms following UV irradiation. Interacts with RNPS1. Interacts with SNAI1. Interacts with PML (isoform PML-12). Interacts with CCAR2.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

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