Substance P Antibody - #DF7522

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製品: Substance P Antibody
カタログ: DF7522
タンパク質の説明: Rabbit polyclonal antibody to Substance P
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IHC
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 16kDa; 15kD(Calculated).
ユニプロット: P20366
RRID: AB_2841021

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
クローナリティ:
Polyclonal
特異性:
Substance P Antibody detects endogenous levels of total Substance P.
RRID:
AB_2841021
引用形式: Affinity Biosciences Cat# DF7522, RRID:AB_2841021.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

C-terminal-flanking peptide; neurokinin A; neurokinin alpha; NKA; NKNA; PPT; preprotachykinin; protachykinin; protachykinin-1; SubstanceP; TAC1; TAC2; Tachykinin 1; Tachykinin precursor 1; Tachykinin1; TKN1_HUMAN; SP;

免疫原

免疫原:

A synthesized peptide derived from human Substance P, corresponding to a region within the internal amino acids.

Uniprot:

P20366(TKN1_HUMAN) >>Visit HPA database.

遺伝子(ID):
TAC1(6863)
タンパク質配列:
MKILVALAVFFLVSTQLFAEEIGANDDLNYWSDWYDSDQIKEELPEPFEHLLQRIARRPKPQQFFGLMGKRDADSSIEKQVALLKALYGHGQISHKRHKTDSFVGLMGKRALNSVAYERSAMQNYERRR

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
88
Xenopus
63
Zebrafish
63
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.

PTMs:

The substance P form is cleaved at Pro-59 by the prolyl endopeptidase FAP (seprase) activity (in vitro).

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the tachykinin family.

参考文献

1). Regulation of macrophages in the dorsal root ganglion through TGF-β1 inhibits painful traumatic neuroma. Journal of translational medicine, 2025 (PubMed: 41121318) [IF=7.4]

Application: WB    Species: Rat    Sample:

Fig. 4 Thermal hyperalgesia measurement and expression of pain-related proteins c-fos and Substance P A, C, D Expression levels of c-fos and Substance P. As pain biomarkers, the expressions of c-fos and SP in dorsal root ganglia (DRG) of the SNT/RAPA group were significantly lower than those in the SNT group (p 
2). The role of substance P in Th17/Treg imbalance and ocular surface damage in chronic allergic conjunctivitis. Journal of translational medicine, 2025 (PubMed: 41327447) [IF=7.4]
3). Terrestrosin D ameliorates skin lesions in an imiquimod-induced psoriasis-like murine model by inhibiting the interaction between Substance P and Dendritic cells. PHYTOMEDICINE, 2022 (PubMed: 34923236) [IF=6.7]
4). Targeting NK-1R attenuates renal fibrosis via modulating inflammatory responses and cell fate in chronic kidney disease. Frontiers in Immunology, 2023 (PubMed: 37033943) [IF=5.7]
5). Astragalus Polysaccharides Ameliorate Diabetic Bladder Dysfunction via Normalization of Neuromuscular Conduction. Journal of agricultural and food chemistry, 2025 (PubMed: 40032633) [IF=5.7]
6). Left superior cervical ganglia lymph node mimicry and its role in rat ventricular arrhythmias following myocardial infarction. Acta physiologica (Oxford, England), 2025 (PubMed: 39803764) [IF=5.6]
7). Multi-omics and network pharmacology approaches reveal the mechanism of action of KeKe tablet against post-infectious cough. Chinese medicine, 2025 (PubMed: 41044788) [IF=5.3]
8). The Effect of Shaoyao Gancao Decoction on Sphincter of Oddi Dysfunction in Hypercholesterolemic Rabbits via Protecting the Enteric Nervous System–Interstitial Cells of Cajal–Smooth Muscle Cells Network. Journal of Inflammation Research, 2021 (PubMed: 34552344) [IF=4.5]
9). Losartan attenuates acetic acid enema-induced visceral hypersensitivity by inhibiting the ACE1/Ang II/AT1 receptor axis in enteric glial cells. European journal of pharmacology, 2023 (PubMed: 36907262) [IF=4.2]
10). Zhiqiao Gancao decoction regulated JAK2/STAT3/ macrophage M1 polarization to ameliorate intervertebral disc degeneration. Heliyon, 2024 (PubMed: 39170327) [IF=4.0]

Application: WB    Species: Rat    Sample:

Fig. 2 The impact of ZQGCD on IDD rats. (A) Paw withdrawal threshold in rats. (B) Paw withdrawal latency in rats. (C) Relative expression level of substance P protein in intervertebral disc tissue of rats. The unedited images are referenced in Fig. S1 (D) Representative HE-stained intervertebral disc sections (magnification: 10 × or 40 × ). (E) Intervertebral disc histopathologic score. *: P < 0.05, **: P < 0.01, ***: P < 0.001, ****: P < 0.0001, ns: P>0.05.
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