CD45 Antibody | Affinity Biosciences

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Western blot analysis of CD45 using HUVEC whole cell lysates.

製品:	CD45 Antibody
カタログ:	DF2912
タンパク質の説明:	Rabbit polyclonal antibody to CD45
アプリケーション:	WB IHC
反応性:	Human, Mouse
予測:	Pig, Dog
分子量:	150kDa; 147kD(Calculated).
ユニプロット:	P08575
RRID:	AB_2840901

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サイズ	価格	在庫状況
100ul	$280	在庫あり
200ul	$350	在庫あり

プロトコル

製品説明

ソース:

Rabbit

アプリケーション:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:

Human,Mouse

予測:

Pig(100%), Dog(100%)

クローナリティ:

Polyclonal

特異性:

CD45 Antibody detects endogenous levels of total CD45.

RRID:

AB_2840901
引用形式: Affinity Biosciences Cat# DF2912, RRID:AB_2840901.

コンジュゲート:

Unconjugated.

精製:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

別名:

折りたたみ／展開

B220; CD 45; CD45; CD45 antigen; CD45R; GP180; L-CA; LCA; Leukocyte common antigen; loc; Ly-5; LY5; Ly5, homolog of; Lyt-4; OTTHUMP00000033813; OTTHUMP00000033816; OTTHUMP00000033817; OTTHUMP00000038574; Protein tyrosine phosphatase receptor type c polypeptide; Protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; Protein tyrosine phosphatase, receptor type, c polypeptide; Ptprc; PTPRC_HUMAN; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen;

免疫原

免疫原:

Uniprot:

P08575(PTPRC_HUMAN) >>Visit HPA database.

遺伝子(ID):

PTPRC(5788)

発現特異性:

P08575 PTPRC_HUMAN:

Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes.

タンパク質配列:

P08575 PTPRC_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTMYLWLKLLAFGFAFLDTEVFVTGQSPTPSPTGLTTAKMPSVPLSSDPLPTHTTAFSPASTFERENDFSETTTSLSPDNTSTQVSPDSLDNASAFNTTGVSSVQTPHLPTHADSQTPSAGTDTQTFSGSAANAKLNPTPGSNAISDVPGERSTASTFPTDPVSPLTTTLSLAHHSSAALPARTSNTTITANTSDAYLNASETTTLSPSGSAVISTTTIATTPSKPTCDEKYANITVDYLYNKETKLFTAKLNVNENVECGNNTCTNNEVHNLTECKNASVSISHNSCTAPDKTLILDVPPGVEKFQLHDCTQVEKADTTICLKWKNIETFTCDTQNITYRFQCGNMIFDNKEIKLENLEPEHEYKCDSEILYNNHKFTNASKIIKTDFGSPGEPQIIFCRSEAAHQGVITWNPPQRSFHNFTLCYIKETEKDCLNLDKNLIKYDLQNLKPYTKYVLSLHAYIIAKVQRNGSAAMCHFTTKSAPPSQVWNMTVSMTSDNSMHVKCRPPRDRNGPHERYHLEVEAGNTLVRNESHKNCDFRVKDLQYSTDYTFKAYFHNGDYPGEPFILHHSTSYNSKALIAFLAFLIIVTSIALLVVLYKIYDLHKKRSCNLDEQQELVERDDEKQLMNVEPIHADILLETYKRKIADEGRLFLAEFQSIPRVFSKFPIKEARKPFNQNKNRYVDILPYDYNRVELSEINGDAGSNYINASYIDGFKEPRKYIAAQGPRDETVDDFWRMIWEQKATVIVMVTRCEEGNRNKCAEYWPSMEEGTRAFGDVVVKINQHKRCPDYIIQKLNIVNKKEKATGREVTHIQFTSWPDHGVPEDPHLLLKLRRRVNAFSNFFSGPIVVHCSAGVGRTGTYIGIDAMLEGLEAENKVDVYGYVVKLRRQRCLMVQVEAQYILIHQALVEYNQFGETEVNLSELHPYLHNMKKRDPPSEPSPLEAEFQRLPSYRSWRTQHIGNQEENKSKNRNSNVIPYDYNRVPLKHELEMSKESEHDSDESSDDDSDSEEPSKYINASFIMSYWKPEVMIAAQGPLKETIGDFWQMIFQRKVKVIVMLTELKHGDQEICAQYWGEGKQTYGDIEVDLKDTDKSSTYTLRVFELRHSKRKDSRTVYQYQYTNWSVEQLPAEPKELISMIQVVKQKLPQKNSSEGNKHHKSTPLLIHCRDGSQQTGIFCALLNLLESAETEEVVDIFQVVKALRKARPGMVSTFEQYQFLYDVIASTYPAQNGQVKKNNHQEDKIEFDNEVDKVKQDANCVNPLGAPEKLPEAKEQAEGSEPTSGTEGPEHSVNGPASPALNQGS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Dog

100

Horse

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08575 基板として

P08575

Site	PTM Type	Source
N234	N-Glycosylation	Uniprot
N278	N-Glycosylation	Uniprot
N286	N-Glycosylation	Uniprot
N337	N-Glycosylation	Uniprot
N421	N-Glycosylation	Uniprot
N490	N-Glycosylation	Uniprot
Y689	Phosphorylation	Uniprot
T860	Phosphorylation	Uniprot
S975	Phosphorylation	Uniprot
Y980	Phosphorylation	Uniprot
S994	Phosphorylation	Uniprot
K1254	Ubiquitination	Uniprot
S1299	Phosphorylation	Uniprot

研究背景

P08575_PTPRC_HUMAN

機能:

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).

(Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

PTMs:

Heavily N- and O-glycosylated.

細胞の位置付け:

Cell membrane>Single-pass type I membrane protein. Membrane raft.
Note: Colocalized with DPP4 in membrane rafts.

組織特異性:

サブユニット構造:

Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1. Interacts with DPP4; the interaction is enhanced in an interleukin-12-dependent manner in activated lymphocytes.

(Microbial infection) Interacts with human cytomegalovirus protein UL11; the interaction is required for binding of UL11 to T-cells.

タンパク質ファミリー:

The first PTPase domain interacts with SKAP1.

Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

· Human Diseases > Immune diseases > Primary immunodeficiency.

· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)

参考文献

1). Sinusoidal endothelial cell progenitor cells promote tumor progression in the patients with hepatocellular carcinoma. Stem Cells International (PubMed: 27313175) [IF=4.5]

Application: IHC Species: Mouse Sample: aorta tissues

Figure 1 CCL19 expression in AS. (A) Detection of CCL19 expression in peripheral blood of patients by ELISA. n=32. (B) qRT-PCR analysis of CCL19 mRNA levels normalized to GAPDH in AS mice aorta and normal aorta tissues. n=10. (C) CCL19 protein levels in the AS or normal mice aorta tissues was performed by western blot. n=4. (D) Representative images of IHC of CCL19 in AS model mice aorta tissues and normal aorta tissues. Bar=50 μm. The effect of different concentrations (E) or different duration (F) of PDGF-BB on the expression of CCL19 was detected by qRT-PCR. n=5. *P<0.05; **P<0.01; ***P<0.001.

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CD45 Antibody - #DF2912

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