OPSB Antibody - #DF10234

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製品: OPSB Antibody
カタログ: DF10234
タンパク質の説明: Rabbit polyclonal antibody to OPSB
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 39 kDa(Observed); 39kD(Calculated).
ユニプロット: P03999
RRID: AB_2840812

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(91%), Bovine(91%), Horse(92%), Sheep(91%), Rabbit(83%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
OPSB Antibody detects endogenous levels of total OPSB.
RRID:
AB_2840812
引用形式: Affinity Biosciences Cat# DF10234, RRID:AB_2840812.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

免疫原

免疫原:

A synthesized peptide derived from human OPSB, corresponding to a region within N-terminal amino acids.

Uniprot:

P03999(OPSB_HUMAN) >>Visit HPA database.

遺伝子(ID):
OPN1SW(611)
発現特異性:
P03999 OPSB_HUMAN:

The three color pigments are found in the cone photoreceptor cells.

タンパク質配列:
MRKMSEEEFYLFKNISSVGPWDGPQYHIAPVWAFYLQAAFMGTVFLIGFPLNAMVLVATLRYKKLRQPLNYILVNVSFGGFLLCIFSVFPVFVASCNGYFVFGRHVCALEGFLGTVAGLVTGWSLAFLAFERYIVICKPFGNFRFSSKHALTVVLATWTIGIGVSIPPFFGWSRFIPEGLQCSCGPDWYTVGTKYRSESYTWFLFIFCFIVPLSLICFSYTQLLRALKAVAAQQQESATTQKAEREVSRMVVVMVGSFCVCYVPYAAFAMYMVNNRNHGLDLRLVTIPSFFSKSACIYNPIIYCFMNKQFQACIMKMVCGKAMTDESDTCSSQKTEVSTVSSTQVGPN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Horse
92
Pig
91
Bovine
91
Sheep
91
Rabbit
83
Xenopus
75
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal (Probable). Required for the maintenance of cone outer segment organization in the ventral retina, but not essential for the maintenance of functioning cone photoreceptors (By similarity). Involved in ensuring correct abundance and localization of retinal membrane proteins (By similarity). May increase spectral sensitivity in dim light (By similarity).

PTMs:

Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.

細胞の位置付け:

Membrane>Multi-pass membrane protein. Photoreceptor inner segment. Cell projection>Cilium>Photoreceptor outer segment.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

The three color pigments are found in the cone photoreceptor cells.

タンパク質ファミリー:

Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

参考文献

1). Exposure to blue light stimulates the proangiogenic capability of exosomes derived from human umbilical cord mesenchymal stem cells. Stem Cell Research & Therapy, 2019 (PubMed: 31779691) [IF=7.5]

Application: WB    Species: Human    Sample: mesenchymal stem cells

Fig. 1 Characterization of MSC photoreceptors for blue/red light irradiation. a Schema of the LED photosystem. b Real-time PCR screening of the basal and stimulated expression of opsins as indicated (OPN4 constitutional expression level was selected as the control group, fold-change = 1). c Proteins were extracted from P2, P4, and P6 passages of MSCs and subjected to immunoblotting. d Proteins were extracted from P4 passage of MSCs with blue/red light illumination and subjected to immunoblotting. e Immunofluorescence staining of the indicated photoreceptors in MSCs

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