製品: CALCRL Antibody
カタログ: DF10203
タンパク質の説明: Rabbit polyclonal antibody to CALCRL
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 53 kDa; 53kD(Calculated).
ユニプロット: Q16602
RRID: AB_2840782

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(86%)
クローナリティ:
Polyclonal
特異性:
CALCRL Antibody detects endogenous levels of total CALCRL.
RRID:
AB_2840782
引用形式: Affinity Biosciences Cat# DF10203, RRID:AB_2840782.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Calcitonin gene related peptide type 1 receptor; Calcitonin receptor like; Calcitonin receptor like receptor; CALCRL; CGRP type 1 receptor; CGRPR; CRLR;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q16602 CALRL_HUMAN:

Predominantly expressed in the lung and heart.

タンパク質配列:
MEKKCTLNFLVLLPFFMILVTAELEESPEDSIQLGVTRNKIMTAQYECYQKIMQDPIQQAEGVYCNRTWDGWLCWNDVAAGTESMQLCPDYFQDFDPSEKVTKICDQDGNWFRHPASNRTWTNYTQCNVNTHEKVKTALNLFYLTIIGHGLSIASLLISLGIFFYFKSLSCQRITLHKNLFFSFVCNSVVTIIHLTAVANNQALVATNPVSCKVSQFIHLYLMGCNYFWMLCEGIYLHTLIVVAVFAEKQHLMWYYFLGWGFPLIPACIHAIARSLYYNDNCWISSDTHLLYIIHGPICAALLVNLFFLLNIVRVLITKLKVTHQAESNLYMKAVRATLILVPLLGIEFVLIPWRPEGKIAEEVYDYIMHILMHFQGLLVSTIFCFFNGEVQAILRRNWNQYKIQFGNSFSNSEALRSASYTVSTISDGPGYSHDCPSEHLNGKSIHDIENVLLKPENLYN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
100
Pig
91
Horse
91
Bovine
91
Sheep
91
Chicken
91
Xenopus
86
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16602 基板として

Site PTM Type Enzyme
S285 Phosphorylation
K403 Ubiquitination
S409 Phosphorylation
S445 Phosphorylation
Y460 Phosphorylation

研究背景

機能:

Receptor for calcitonin-gene-related peptide (CGRP) together with RAMP1 and receptor for adrenomedullin together with RAMP3 (By similarity). Receptor for adrenomedullin together with RAMP2. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Predominantly expressed in the lung and heart.

サブユニット構造:

Heterodimer of CALCRL and RAMP3 (By similarity). Heterodimer of CALCRL and RAMP1 or CALCRL and RAMP2.

タンパク質ファミリー:

Belongs to the G-protein coupled receptor 2 family.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

参考文献

1). Intermedin in Paraventricular Nucleus Attenuates Ang II-Induced Sympathoexcitation through the Inhibition of NADPH Oxidase-Dependent ROS Generation in Obese Rats with Hypertension. International Journal of Molecular Sciences (PubMed: 31466304) [IF=5.6]

Application: WB    Species: rat    Sample: paraventricular nucleus

Figure 1. |Plasma norepinephrine (NE) level (A. n = 6 or 7), systolic blood pressure (B. n = 6–7); and relative values of intermedin (IMD), calcitonin receptor–like receptor (CRLR) and receptor activity–modifying protein (RAMP) 1, RAMP2, and RAMP3 protein expression in the paraventricular nucleus (PVN) in control and obesity-related hypertensive (OH) rats (C–H. n = 3–4). Values are mean± SEM. * p < 0.05 versus control.

2). Exogenous CGRP Regulates Apoptosis and Autophagy to Alleviate Traumatic Brain Injury Through Akt/mTOR Signalling Pathway. NEUROCHEMICAL RESEARCH (PubMed: 33063244) [IF=4.4]

Application: WB    Species: mouse    Sample: brain cortical

Fig. 1 CGRP alleviated the brain lesion induced by TBI. a Relative protein expression of CGRP in the brain cortical tissues with or without CGRP administration after TBI over time was detected by western blot (loading volume of CGRP: 15 μg).

3). Adrenomedullin ameliorates palmitic acid-induced insulin resistance through PI3K/Akt pathway in adipocytes. ACTA DIABETOLOGICA (PubMed: 34978596) [IF=3.8]

4). Correlation of vascular change with TRPV1, TRPV4, and TRPA1 in a rat model of inferior gluteal artery perforator flap. WOUND REPAIR AND REGENERATION (PubMed: 35384152) [IF=2.9]

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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