製品: TSSK1 Antibody
カタログ: DF10144
タンパク質の説明: Rabbit polyclonal antibody to TSSK1
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Dog
分子量: 42 kDa; 42kD(Calculated).
ユニプロット: Q9BXA7
RRID: AB_2840724

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 100ul $280 在庫あり
 200ul $350 在庫あり

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製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(85%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
TSSK1 Antibody detects endogenous levels of total TSSK1.
RRID:
AB_2840724
引用形式: Affinity Biosciences Cat# DF10144, RRID:AB_2840724.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

FKSG81; serine/threonine kinase 22D (spermiogenesis associated); serine/threonine kinase FKSG81; Serine/threonine-protein kinase 22A; spermiogenesis associated 4; SPOGA1; SPOGA4; STK22A; STK22D; Testis specific kinase 1; Testis specific serine kinase 1; testis specific serine kinase 1B; Testis-specific kinase 1; Testis-specific serine/threonine-protein kinase 1; TSK 1; TSK-1; TSK1; TSSK-1; TSSK1_HUMAN; Tssk1b;

免疫原

免疫原:

A synthesized peptide derived from human TSSK1, corresponding to a region within the internal amino acids.

Uniprot:
遺伝子(ID):
発現特異性:
Q9BXA7 TSSK1_HUMAN:

Testis-specific. Present in sperm (at protein level).

タンパク質配列:
MDDAAVLKRRGYLLGINLGEGSYAKVKSAYSERLKFNVAIKIIDRKKAPADFLEKFLPREIEILAMLNHCSIIKTYEIFETSHGKVYIVMELAVQGDLLELIKTRGALHEDEARKKFHQLSLAIKYCHDLDVVHRDLKCDNLLLDKDFNIKLSDFSFSKRCLRDDSGRMALSKTFCGSPAYAAPEVLQGIPYQPKVYDIWSLGVILYIMVCGSMPYDDSNIKKMLRIQKEHRVNFPRSKHLTGECKDLIYHMLQPDVNRRLHIDEILSHCWMQPKARGSPSVAINKEGESSRGTEPLWTPEPGSDKKSATKLEPEGEAQPQAQPETKPEGTAMQMSRQSEILGFPSKPSTMETEEGPPQQPPETRAQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Sheep
85
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Testis-specific serine/threonine-protein kinase required during spermatid development. Phosphorylates 'Ser-288' of TSKS. Involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm. During spermatogenesis, required for the transformation of a ring-shaped structure around the base of the flagellum originating from the chromatoid body.

PTMs:

Autophosphorylated.

Ubiquitinated; HSP90 activity negatively regulates ubiquitination and degradation.

細胞の位置付け:

Cytoplasm. Cytoplasmic vesicle>Secretory vesicle>Acrosome. Cell projection>Cilium>Flagellum.
Note: In spermatozoa, present in the sperm head and in the flagellum.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Testis-specific. Present in sperm (at protein level).

タンパク質ファミリー:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

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