製品: MYCBP Antibody
カタログ: DF10076
タンパク質の説明: Rabbit polyclonal antibody to MYCBP
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse
予測: Bovine, Rabbit, Dog, Chicken, Xenopus
分子量: 12 kDa(Observed); 12kD(Calculated).
ユニプロット: Q99417
RRID: AB_2840656

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Bovine(100%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(91%)
クローナリティ:
Polyclonal
特異性:
MYCBP Antibody detects endogenous levels of total MYCBP.
RRID:
AB_2840656
引用形式: Affinity Biosciences Cat# DF10076, RRID:AB_2840656.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

AMY 1; AMY-1; AMY1; Associate of Myc 1; C myc binding protein; C-Myc-binding protein; Cmyc binding protein; MYCBP; MYCBP_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human MYCBP, corresponding to a region within N-terminal amino acids.

Uniprot:
遺伝子(ID):
発現特異性:
Q99417 MYCBP_HUMAN:

Highly expressed in heart, placenta, pancreas, skeletal muscle and kidney. Also present at low levels in lung.

タンパク質配列:
MAHYKAADSKREQFRRYLEKSGVLDTLTKVLVALYEEPEKPNSALDFLKHHLGAATPENPEIELLRLELAEMKEKYEAIVEENKKLKAKLAQYEPPQEEKRAE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Dog
100
Rabbit
100
Xenopus
91
Chicken
91
Pig
0
Horse
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

May control the transcriptional activity of MYC. Stimulates the activation of E box-dependent transcription by MYC.

細胞の位置付け:

Cytoplasm. Nucleus. Mitochondrion.
Note: Translocates into the nucleus in the S phase of the cell cycle upon an increase of MYC expression. Found in the mitochondria when associated with AKAP1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in heart, placenta, pancreas, skeletal muscle and kidney. Also present at low levels in lung.

タンパク質ファミリー:

Belongs to the AMY1 family.

参考文献

1). Proteomic profiling of serum extracellular vesicles identifies diagnostic markers for echinococcosis. PLOS Neglected Tropical Diseases, 2017 (PubMed: 36206314) [IF=3.4]

Application: WB    Species: Mouse    Sample:

Fig 1. The dynamic changes of mouse proteins in serum-derived exosomes in response to E. multilocularis infection. (A) and (B) Transmission electron microscopy of the enriched extracellular vesicles (EVs) isolated from the sera of E. multilocularis-infected (A) and uninfected mice (B). (C) The diameter distribution of the purified EVs. (D) Western blotting analysis of mammalian EV-marker CD63 in the purified EVs. (E) Heatmap of differentially abundance proteins between the serum exosomes from E. multilocularis-infected and uninfected mice. (F) Western blotting analysis the abundance of TGF-β1, GPATC8, LRP1, and AMY1 during E. multilocularis infection. https://doi.org/10.1371/journal.pntd.0010814.g001

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