製品: Phospho-RCC1 (Ser11) Antibody
カタログ: AF8507
タンパク質の説明: Rabbit polyclonal antibody to Phospho-RCC1 (Ser11)
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 45 kDa; 45kD(Calculated).
ユニプロット: P18754
RRID: AB_2840561

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(89%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-RCC1 (Ser11) Antibody detects endogenous levels of RCC1 only when phosphorylated at Ser11.
RRID:
AB_2840561
引用形式: Affinity Biosciences Cat# AF8507, RRID:AB_2840561.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Cell cycle regulatory protein; CHC 1; CHC1; Chromosome condensation 1; Chromosome condensation protein 1; Guanine nucleotide releasing protein; HERC2; Ran GEF; RanGEF; RCC 1; RCC1; RCC1 I; RCC1_HUMAN; Regulator of chromosome condensation 1; Regulator of chromosome condensation; SHEP1; SNHG3 RCC1; SNHG3 RCC1 readthrough transcript;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MSPKRIAKRRSPPADAIPKSKKVKVSHRSHSTEPGLVLTLGQGDVGQLGLGENVMERKKPALVSIPEDVVQAEAGGMHTVCLSKSGQVYSFGCNDEGALGRDTSVEGSEMVPGKVELQEKVVQVSAGDSHTAALTDDGRVFLWGSFRDNNGVIGLLEPMKKSMVPVQVQLDVPVVKVASGNDHLVMLTADGDLYTLGCGEQGQLGRVPELFANRGGRQGLERLLVPKCVMLKSRGSRGHVRFQDAFCGAYFTFAISHEGHVYGFGLSNYHQLGTPGTESCFIPQNLTSFKNSTKSWVGFSGGQHHTVCMDSEGKAYSLGRAEYGRLGLGEGAEEKSIPTLISRLPAVSSVACGASVGYAVTKDGRVFAWGMGTNYQLGTGQDEDAWSPVEMMGKQLENRVVLSVSSGGQHTVLLVKDKEQS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Sheep
89
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P18754 基板として

Site PTM Type Enzyme
S2 Methylation
S2 Phosphorylation Q13188 (STK3) , P06493 (CDK1)
S11 Phosphorylation P06493 (CDK1) , Q13188 (STK3)
S20 Phosphorylation
S26 Phosphorylation
S29 Phosphorylation
T32 Phosphorylation
S85 Phosphorylation
Y89 Phosphorylation
S90 Phosphorylation
C93 S-Nitrosylation
S104 Phosphorylation
K114 Sumoylation
K114 Ubiquitination
K120 Ubiquitination
T131 Phosphorylation
T135 Phosphorylation
K160 Ubiquitination
K161 Ubiquitination
S162 Phosphorylation
R214 Methylation
K227 Ubiquitination
K232 Ubiquitination
T274 Phosphorylation P06493 (CDK1)
T306 Phosphorylation
S311 Phosphorylation
K335 Sumoylation
K335 Ubiquitination
S336 Phosphorylation
T339 Phosphorylation
K362 Ubiquitination
S387 Phosphorylation P06493 (CDK1)
S403 Phosphorylation
S405 Phosphorylation
S406 Phosphorylation
T411 Phosphorylation

研究背景

機能:

Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis. Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis. Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA.

PTMs:

N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di- and trimethylation produce a permanent positive charge on the amino group, which facilitates electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.

細胞の位置付け:

Nucleus. Chromosome. Cytoplasm.
Note: Predominantly nuclear in interphase cells (PubMed:12194828). Binds to mitotic chromosomes (PubMed:12194828, PubMed:17435751, PubMed:20668449).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with RAN. Interacts (via N-terminus and RCC1 repeats) with KPNA4. Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation.

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