製品: Phospho-EIF6 (Ser235) Antibody
カタログ: AF8474
タンパク質の説明: Rabbit polyclonal antibody to Phospho-EIF6 (Ser235)
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 27kDa; 27kD(Calculated).
ユニプロット: P56537
RRID: AB_2840528

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(89%), Dog(100%), Xenopus(89%)
クローナリティ:
Polyclonal
特異性:
Phospho-EIF6 (Ser235) Antibody detects endogenous levels of EIF6 only when phosphorylated at Ser235.
RRID:
AB_2840528
引用形式: Affinity Biosciences Cat# AF8474, RRID:AB_2840528.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

b(2)gcn; B(2)GCN homolog; B4 integrin interactor; Binding protein of beta-4 integrin; CAB; eIF-6; EIF3A; EIF6; Eukaryotic translation initiation factor 3A; Eukaryotic translation initiation factor 6; IF6_HUMAN; ITGB4BP; OK/SW-cl.27; p27 beta 4 integrin binding protein; p27(BBP); p27BBP; RP4-614O4.1;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P56537 IF6_HUMAN:

Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).

タンパク質配列:
MAVRASFENNCEIGCFAKLTNTYCLVAIGGSENFYSVFEGELSDTIPVVHASIAGCRIIGRMCVGNRHGLLVPNNTTDQELQHIRNSLPDTVQIRRVEERLSALGNVTTCNDYVALVHPDLDRETEEILADVLKVEVFRQTVADQVLVGSYCVFSNQGGLVHPKTSIEDQDELSSLLQVPLVAGTVNRGSEVIAAGMVVNDWCAFCGLDTTSTELSVVESVFKLNEAQPSTIATSMRDSLIDSLT

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
89
Rabbit
89
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P56537 基板として

Site PTM Type Enzyme
S6 Phosphorylation
C11 S-Nitrosylation
C15 S-Nitrosylation
R85 Methylation
R95 Methylation
Y113 Phosphorylation
S150 Phosphorylation
S174 Phosphorylation P48729 (CSNK1A1)
S175 Phosphorylation P48729 (CSNK1A1)
T185 Phosphorylation
K223 Acetylation
S230 Phosphorylation
T231 Phosphorylation
T234 Phosphorylation
S235 Phosphorylation P17252 (PRKCA) , P05771 (PRKCB)
S239 Phosphorylation
S243 Phosphorylation
T245 Phosphorylation

研究背景

機能:

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis (By similarity). Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC. Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth (By similarity).

PTMs:

Phosphorylation at Ser-174 and Ser-175 by CSNK1D/CK1 promotes nuclear export.

細胞の位置付け:

Cytoplasm. Nucleus>Nucleolus.
Note: Shuttles between cytoplasm and nucleus/nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).

サブユニット構造:

Monomer. Associates with the 60S ribosomal subunit. Interacts with RACK1. Interacts with DICER1, AGO2, TARBP2, MOV10 and RPL7A; they form a large RNA-induced silencing complex (RISC).

タンパク質ファミリー:

Belongs to the eIF-6 family.

研究領域

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

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