製品: Phospho-EPHB3 (Tyr608) Antibody
カタログ: AF8431
タンパク質の説明: Rabbit polyclonal antibody to Phospho-EPHB3 (Tyr608)
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 110kDa; 110kD(Calculated).
ユニプロット: P54753
RRID: AB_2840488

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(88%), Chicken(100%), Xenopus(88%)
クローナリティ:
Polyclonal
特異性:
Phospho-EPHB3 (Tyr608) Antibody detects endogenous levels of EPHB3 only when phosphorylated at Tyr608.
RRID:
AB_2840488
引用形式: Affinity Biosciences Cat# AF8431, RRID:AB_2840488.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Cek10; EK2; Embryonic kinase 2; EPH Like Tyrosine Kinase 2; EPH receptor B3; EPH-like kinase 2; ephb3; EPHB3_HUMAN; Ephrin receptor EphB3; Ephrin type B receptor 3; Ephrin type-B receptor 3; ETK2; hEK2; Human Embryo Kinase 2; Mdk5; Sek4; TYRO6; Tyrosine protein kinase receptor HEK2; Tyrosine protein kinase TYRO6; Tyrosine-protein kinase TYRO6;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P54753 EPHB3_HUMAN:

Ubiquitous.

タンパク質配列:
MARARPPPPPSPPPGLLPLLPPLLLLPLLLLPAGCRALEETLMDTKWVTSELAWTSHPESGWEEVSGYDEAMNPIRTYQVCNVRESSQNNWLRTGFIWRRDVQRVYVELKFTVRDCNSIPNIPGSCKETFNLFYYEADSDVASASSPFWMENPYVKVDTIAPDESFSRLDAGRVNTKVRSFGPLSKAGFYLAFQDQGACMSLISVRAFYKKCASTTAGFALFPETLTGAEPTSLVIAPGTCIPNAVEVSVPLKLYCNGDGEWMVPVGACTCATGHEPAAKESQCRPCPPGSYKAKQGEGPCLPCPPNSRTTSPAASICTCHNNFYRADSDSADSACTTVPSPPRGVISNVNETSLILEWSEPRDLGGRDDLLYNVICKKCHGAGGASACSRCDDNVEFVPRQLGLTERRVHISHLLAHTRYTFEVQAVNGVSGKSPLPPRYAAVNITTNQAAPSEVPTLRLHSSSGSSLTLSWAPPERPNGVILDYEMKYFEKSEGIASTVTSQMNSVQLDGLRPDARYVVQVRARTVAGYGQYSRPAEFETTSERGSGAQQLQEQLPLIVGSATAGLVFVVAVVVIAIVCLRKQRHGSDSEYTEKLQQYIAPGMKVYIDPFTYEDPNEAVREFAKEIDVSCVKIEEVIGAGEFGEVCRGRLKQPGRREVFVAIKTLKVGYTERQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMILTEFMENCALDSFLRLNDGQFTVIQLVGMLRGIAAGMKYLSEMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDPSDPTYTSSLGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVINAVEQDYRLPPPMDCPTALHQLMLDCWVRDRNLRPKFSQIVNTLDKLIRNAASLKVIASAQSGMSQPLLDRTVPDYTTFTTVGDWLDAIKMGRYKESFVSAGFASFDLVAQMTAEDLLRIGVTLAGHQKKILSSIQDMRLQMNQTLPVQV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Chicken
100
Rabbit
100
Dog
88
Xenopus
88
Zebrafish
88
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P54753 基板として

Site PTM Type Enzyme
S167 Phosphorylation
S201 Phosphorylation
S204 Phosphorylation
S249 Phosphorylation
S413 Phosphorylation
T448 Phosphorylation
T458 Phosphorylation
Y593 Phosphorylation
T594 Phosphorylation
Y600 Phosphorylation
Y608 Phosphorylation
T613 Phosphorylation
Y614 Phosphorylation P54753 (EPHB3)
T666 Phosphorylation
T731 Phosphorylation
Y754 Phosphorylation
S768 Phosphorylation
S775 Phosphorylation
S780 Phosphorylation
S788 Phosphorylation
T791 Phosphorylation
Y792 Phosphorylation
S794 Phosphorylation
S795 Phosphorylation
Y812 Phosphorylation
T891 Phosphorylation
S901 Phosphorylation
Y924 Phosphorylation
T971 Phosphorylation

PTMs - P54753 酵素として

Substrate Site Source
P32004 (L1CAM) Y1229 Uniprot
P54753 (EPHB3) Y614 Uniprot

研究背景

機能:

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.

PTMs:

Phosphorylated. Autophosphorylates upon ligand-binding. Autophosphorylation on Tyr-614 is required for interaction with SH2 domain-containing proteins.

細胞の位置付け:

Cell membrane>Single-pass type I membrane protein. Cell projection>Dendrite.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous.

サブユニット構造:

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity).

タンパク質ファミリー:

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

研究領域

· Organismal Systems > Development > Axon guidance.   (View pathway)

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