Phospho-IRF5 (Ser437) Antibody - #AF8382

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製品: Phospho-IRF5 (Ser437) Antibody
カタログ: AF8382
タンパク質の説明: Rabbit polyclonal antibody to Phospho-IRF5 (Ser437)
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 56kDa; 56kD(Calculated).
ユニプロット: Q13568
RRID: AB_2840443

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-IRF5 (Ser437) Antibody detects endogenous levels of IRF5 only when phosphorylated at Ser437.
RRID:
AB_2840443
引用形式: Affinity Biosciences Cat# AF8382, RRID:AB_2840443.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Interferon regulatory factor 5; Interferon regulatory factor 5 bone marrow variant; IRF 5; IRF-5; Irf5; IRF5_HUMAN; SLEB10;

免疫原

免疫原:
Uniprot:

Q13568(IRF5_HUMAN) >>Visit HPA database.

遺伝子(ID):
IRF5(3663)
タンパク質配列:
MNQSIPVAPTPPRRVRLKPWLVAQVNSCQYPGLQWVNGEKKLFCIPWRHATRHGPSQDGDNTIFKAWAKETGKYTEGVDEADPAKWKANLRCALNKSRDFRLIYDGPRDMPPQPYKIYEVCSNGPAPTDSQPPEDYSFGAGEEEEEEEELQRMLPSLSLTEDVKWPPTLQPPTLRPPTLQPPTLQPPVVLGPPAPDPSPLAPPPGNPAGFRELLSEVLEPGPLPASLPPAGEQLLPDLLISPHMLPLTDLEIKFQYRGRPPRALTISNPHGCRLFYSQLEATQEQVELFGPISLEQVRFPSPEDIPSDKQRFYTNQLLDVLDRGLILQLQGQDLYAIRLCQCKVFWSGPCASAHDSCPNPIQREVKTKLFSLEHFLNELILFQKGQTNTPPPFEIFFCFGEEWPDRKPREKKLITVQVVPVAARLLLEMFSGELSWSADSIRLQISNPDLKDRMVEQFKELHHIWQSQQRLQPVAQAPPGAGLGVGQGPWPMHPAGMQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
90
Chicken
80
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13568 基板として

Site PTM Type Enzyme
T10 Phosphorylation
Y136 Phosphorylation
S158 Phosphorylation Q9UHD2 (TBK1)
S293 Phosphorylation Q9UHD2 (TBK1)
S301 Phosphorylation
K309 Ubiquitination
Y313 Phosphorylation
Y335 Phosphorylation
K411 Ubiquitination
K412 Ubiquitination
S435 Phosphorylation O43353 (RIPK2)
S437 Phosphorylation
S440 Phosphorylation
S446 Phosphorylation O14920 (IKBKB) , O43353 (RIPK2)

研究背景

機能:

Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling.

PTMs:

Phosphorylation of serine and threonine residues in a C-terminal autoinhibitory region, stimulates dimerization, transport into the nucleus, assembly with the coactivator CBP/p300 and initiation of transcription.

'Lys-63'-linked polyubiquitination by TRAF6 is required for activation.

細胞の位置付け:

Cytoplasm. Nucleus.
Note: Shuttles between the nucleus and the cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homodimer, when phosphorylated.

タンパク質ファミリー:

Belongs to the IRF family.

研究領域

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

参考文献

1). Mitochondrial DNA mediates immunoparalysis of dendritic cells in sepsis via STING signalling. CELL PROLIFERATION (PubMed: 36106559) [IF=8.5]

Application: WB    Species: Mice    Sample: BMDCs

FIGURE 4 Cytoplasmic mtDNA markedly activated STING signalling. BMDCs were treated with mtDNA (10 μg/ml), or transfected with vehicle or mtDNA (10 μg/ml) for 24 h, then were harvested for western blot and qPCR. (A) Representative blots and relative quantification protein levels of STING pathway (n = 3). (B) qPCR quantitation of IFN‐β (n = 7). (C) Representative images of nuclear translocation of IRF3 in BMDC treated as indicated. Scale bar: 5 μm. (D) Representative blots and relative quantification protein levels of TLR9 pathway (n = 3). (E) qPCR quantitation of TNF‐α, IL‐6 and VCAM‐1 (n = 6–7). (F) Representative blots and relative quantification protein levels of NLRP3 pathway (n = 3). (G) qPCR quantitation of IL‐1β, IL‐18 (n = 7). Data are shown as the mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. BMDC, bone marrow derived dendritic cell; IFN, interferon; mtDNA, mitochondrial DNA; mtDNA (T), mtDNA transfection; Veh (T), vehicle transfection.
2). miR-31-5p Regulates Type I Interferon by Targeting SLC15A4 in Plasmacytoid Dendritic Cells of Systemic Lupus Erythematosus. Journal of Inflammation Research (PubMed: 36510495) [IF=4.5]

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