NRIP2 Antibody - #AF0597

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製品: NRIP2 Antibody
カタログ: AF0597
タンパク質の説明: Rabbit polyclonal antibody to NRIP2
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human, Mouse
予測: Horse
分子量: 25kDa; 31kD(Calculated).
ユニプロット: Q9BQI9
RRID: AB_2834307

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Horse(80%)
クローナリティ:
Polyclonal
特異性:
NRIP2 Antibody detects endogenous levels of total NRIP2.
RRID:
AB_2834307
引用形式: Affinity Biosciences Cat# AF0597, RRID:AB_2834307.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

DKFZp761G1913;; Neuronal interacting factor X 1; Nix1; Nrip2; NRIP2_HUMAN; Nuclear receptor interacting protein 2; Nuclear receptor-interacting protein 2;

免疫原

免疫原:

A synthesized peptide derived from human NRIP2, corresponding to a region within N-terminal amino acids.

Uniprot:

Q9BQI9(NRIP2_HUMAN) >>Visit HPA database.

遺伝子(ID):
NRIP2(83714)
タンパク質の説明:
NRIP2 Down-regulates transcriptional activation by nuclear receptors such as NR1F2. Interacts with NR1F2, RARA and THRB in a ligand-dependent manner.
タンパク質配列:
MLFIFPLSLPWRPSCWKESCSTGQRQAGRSREDSVTPPPSSPWPTPPAGAMSTKQEARRDEGEARTRGQEAQLRDRAHLSQQRRLKQATQFLHKDSADLLPLDSLKRLGTSKDLQPRSVIQRRLVEGNPNWLQGEPPRMQDLIHGQESRRKTSRTEIPALLVNCKCQDQLLRVAVDTGTQYNRISAGCLSRLGLEKRVLKASAGDLAPGPPTQVEQLELQLGQETVVCSAQVVDAESPEFCLGLQTLLSLKCCIDLEHGVLRLKAPFSELPFLPLYQEPGQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
80
Bovine
67
Sheep
67
Rabbit
67
Pig
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Down-regulates transcriptional activation by nuclear receptors such as NR1F2.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

参考文献

1). Nuclear Receptor Interacting Protein-2 Mediates the Stabilization and Activation of β-Catenin During Podocyte Injury. Frontiers in cell and developmental biology, 2021 (PubMed: 35004680) [IF=4.6]

Application: WB    Species: human    Sample:

FIGURE 1. NRIP2 binds to β-catenin in podocytes. (A) Coimmunoprecipitation (co-IP) of endogenous NRIP2 with β-catenin in podocytes. β-Catenin was immunoprecipitated, and the amount of NRIP2 bound to β-catenin was determined using immunoblotting analysis. Cells transfected with siRNAs targeting NRIP2 were used as negative controls. (B) The N-terminal domain of β-catenin mediates the interaction of β-catenin with NRIP2. Schematic illustration of full-length β-catenin and β-catenin mutants. Myc-tagged β-catenin and its deletion mutants lacking the C-terminus, N-terminus and armadillo domain were co-transfected with HA-NRIP2 in podocytes. After 24 h of transfection, the cells were lysed and subjected to IP with HA beads followed by IB with anti-Myc and anti-HA antibodies. FL, β-catenin full length; N+C, β-catenin full length lacking Armadillo domain; N+A, β-catenin full length lacking C terminus; N, β-catenin full length lacking both Armadillo domain and C terminus.

Application: IF/ICC    Species: human    Sample:

FIGURE 1. NRIP2 binds to β-catenin in podocytes. (A) Coimmunoprecipitation (co-IP) of endogenous NRIP2 with β-catenin in podocytes. β-Catenin was immunoprecipitated, and the amount of NRIP2 bound to β-catenin was determined using immunoblotting analysis. Cells transfected with siRNAs targeting NRIP2 were used as negative controls. (B) The N-terminal domain of β-catenin mediates the interaction of β-catenin with NRIP2. Schematic illustration of full-length β-catenin and β-catenin mutants. Myc-tagged β-catenin and its deletion mutants lacking the C-terminus, N-terminus and armadillo domain were co-transfected with HA-NRIP2 in podocytes. After 24 h of transfection, the cells were lysed and subjected to IP with HA beads followed by IB with anti-Myc and anti-HA antibodies. FL, β-catenin full length; N+C, β-catenin full length lacking Armadillo domain; N+A, β-catenin full length lacking C terminus; N, β-catenin full length lacking both Armadillo domain and C terminus.

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