製品: Phospho-JAK3 (Tyr904) Antibody
カタログ: AF8159
タンパク質の説明: Rabbit polyclonal antibody to Phospho-JAK3 (Tyr904)
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Bovine, Sheep, Dog
分子量: 125kDa; 125kD(Calculated).
ユニプロット: P52333
RRID: AB_2840221

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Sheep(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-JAK3 (Tyr904) Antibody detects endogenous levels of JAK3 only when phosphorylated at Tyr904.
RRID:
AB_2840221
引用形式: Affinity Biosciences Cat# AF8159, RRID:AB_2840221.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

EC 2.7.10.2; JAK 3; JAK L; JAK-3; Jak3; JAK3 HUMAN; JAK3_HUMAN; JAKL; Janus kinase 3 (a protein tyrosine kinase, leukocyte); Janus kinase 3; Janus Kinase3; L JAK; L-JAK; Leukocyte janus kinase; LJAK; Protein tyrosine kinase leukocyte; Tyrosine protein kinase JAK3; Tyrosine-protein kinase JAK3;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P52333 JAK3_HUMAN:

In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins.

タンパク質配列:
MAPPSEETPLIPQRSCSLLSTEAGALHVLLPARGPGPPQRLSFSFGDHLAEDLCVQAAKASGILPVYHSLFALATEDLSCWFPPSHIFSVEDASTQVLLYRIRFYFPNWFGLEKCHRFGLRKDLASAILDLPVLEHLFAQHRSDLVSGRLPVGLSLKEQGECLSLAVLDLARMAREQAQRPGELLKTVSYKACLPPSLRDLIQGLSFVTRRRIRRTVRRALRRVAACQADRHSLMAKYIMDLERLDPAGAAETFHVGLPGALGGHDGLGLLRVAGDGGIAWTQGEQEVLQPFCDFPEIVDISIKQAPRVGPAGEHRLVTVTRTDNQILEAEFPGLPEALSFVALVDGYFRLTTDSQHFFCKEVAPPRLLEEVAEQCHGPITLDFAINKLKTGGSRPGSYVLRRSPQDFDSFLLTVCVQNPLGPDYKGCLIRRSPTGTFLLVGLSRPHSSLRELLATCWDGGLHVDGVAVTLTSCCIPRPKEKSNLIVVQRGHSPPTSSLVQPQSQYQLSQMTFHKIPADSLEWHENLGHGSFTKIYRGCRHEVVDGEARKTEVLLKVMDAKHKNCMESFLEAASLMSQVSYRHLVLLHGVCMAGDSTMVQEFVHLGAIDMYLRKRGHLVPASWKLQVVKQLAYALNYLEDKGLPHGNVSARKVLLAREGADGSPPFIKLSDPGVSPAVLSLEMLTDRIPWVAPECLREAQTLSLEADKWGFGATVWEVFSGVTMPISALDPAKKLQFYEDRQQLPAPKWTELALLIQQCMAYEPVQRPSFRAVIRDLNSLISSDYELLSDPTPGALAPRDGLWNGAQLYACQDPTIFEERHLKYISQLGKGNFGSVELCRYDPLGDNTGALVAVKQLQHSGPDQQRDFQREIQILKALHSDFIVKYRGVSYGPGRQSLRLVMEYLPSGCLRDFLQRHRARLDASRLLLYSSQICKGMEYLGSRRCVHRDLAARNILVESEAHVKIADFGLAKLLPLDKDYYVVREPGQSPIFWYAPESLSDNIFSRQSDVWSFGVVLYELFTYCDKSCSPSAEFLRMMGCERDVPALCRLLELLEEGQRLPAPPACPAEVHELMKLCWAPSPQDRPSFSALGPQLDMLWSGSRGCETHAFTAHPEGKHHSLSFS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Zebrafish
78
Pig
0
Horse
0
Xenopus
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52333 基板として

Site PTM Type Enzyme
S5 Phosphorylation
T8 Phosphorylation
S15 Phosphorylation
S17 Phosphorylation
S20 Phosphorylation
K122 Ubiquitination
K157 Ubiquitination
K186 Ubiquitination
K237 Ubiquitination
K361 Ubiquitination
K388 Ubiquitination
K390 Ubiquitination
T391 Phosphorylation
S398 Phosphorylation
K482 Ubiquitination
S498 Phosphorylation
Y506 Phosphorylation
K534 Ubiquitination
Y536 Phosphorylation
S568 Phosphorylation
K624 Ubiquitination
K652 Ubiquitination
S663 Phosphorylation
K734 Ubiquitination
K748 Acetylation
S782 Phosphorylation
Y785 Phosphorylation P52333 (JAK3)
K823 Ubiquitination
K830 Ubiquitination
K855 Ubiquitination
K876 Ubiquitination
S880 Phosphorylation
Y886 Phosphorylation
Y904 Phosphorylation P52333 (JAK3)
Y929 Phosphorylation
S931 Phosphorylation
K935 Ubiquitination
Y939 Phosphorylation P52333 (JAK3)
S959 Phosphorylation
K972 Ubiquitination
K978 Ubiquitination
Y980 Phosphorylation P52333 (JAK3)
Y981 Phosphorylation P52333 (JAK3)
K1117 Ubiquitination
S1120 Phosphorylation

PTMs - P52333 酵素として

Substrate Site Source
O14939 (PLD2) Y415 Uniprot
O95644 (NFATC1) Y371 Uniprot
P23458 (JAK1) Y1034 Uniprot
P23458 (JAK1) Y1035 Uniprot
P29353-2 (SHC1) Y410 Uniprot
P29353-2 (SHC1) Y448 Uniprot
P42224 (STAT1) Y701 Uniprot
P42229 (STAT5A) Y694 Uniprot
P52333 (JAK3) Y785 Uniprot
P52333 (JAK3) Y904 Uniprot
P52333 (JAK3) Y939 Uniprot
P52333-1 (JAK3) Y980 Uniprot
P52333 (JAK3) Y981 Uniprot
Q15717 (ELAVL1) Y63 Uniprot
Q15717 (ELAVL1) Y68 Uniprot
Q15717 (ELAVL1) Y200 Uniprot
Q96LC7 (SIGLEC10) Y597 Uniprot
Q96LC7 (SIGLEC10) Y667 Uniprot
Q96LC7 (SIGLEC10) Y691 Uniprot

研究背景

機能:

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.

PTMs:

Tyrosine phosphorylated in response to IL-2 and IL-4. Dephosphorylation of Tyr-980 and Tyr-981 by PTPN2 negatively regulates cytokine-mediated signaling (Probable).

細胞の位置付け:

Endomembrane system>Peripheral membrane protein. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins.

サブユニット構造:

Interacts with STAM2 and MYO18A (By similarity). Interacts with SHB.

タンパク質ファミリー:

Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

研究領域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Immune diseases > Primary immunodeficiency.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

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