OGR1/GPR68 Antibody - #AF0723

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製品: OGR1/GPR68 Antibody
カタログ: AF0723
タンパク質の説明: Rabbit polyclonal antibody to OGR1/GPR68
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human, Mouse
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 40~70 kD; 41kD(Calculated).
ユニプロット: Q15743
RRID: AB_2834264

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
OGR1/GPR68 Antibody detects endogenous levels of total OGR1/GPR68.
RRID:
AB_2834264
引用形式: Affinity Biosciences Cat# AF0723, RRID:AB_2834264.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

G protein coupled receptor 68; G-protein coupled receptor 68; GPR 12A; GPR 68; GPR12A; Gpr68; MGC111379; MGC156983; OGR 1; OGR-1; OGR1; OGR1_HUMAN; Ovarian cancer G protein coupled receptor 1; Ovarian cancer G-protein coupled receptor 1; Sphingosylphosphorylcholine receptor;

免疫原

免疫原:

A synthesized peptide derived from human OGR1/GPR68, corresponding to a region within the internal amino acids.

Uniprot:

Q15743(OGR1_HUMAN) >>Visit HPA database.

遺伝子(ID):
GPR68(8111)
発現特異性:
Q15743 OGR1_HUMAN:

Found at low level in a wide range of tissues, but significantly expressed in lung, kidney, bone and nervous system.

タンパク質の説明:
Protein type: Secreted; Secreted, signal peptide Cellular Component: extracellular region
タンパク質配列:
MGNITADNSSMSCTIDHTIHQTLAPVVYVTVLVVGFPANCLSLYFGYLQIKARNELGVYLCNLTVADLFYICSLPFWLQYVLQHDNWSHGDLSCQVCGILLYENIYISVGFLCCISVDRYLAVAHPFRFHQFRTLKAAVGVSVVIWAKELLTSIYFLMHEEVIEDENQHRVCFEHYPIQAWQRAINYYRFLVGFLFPICLLLASYQGILRAVRRSHGTQKSRKDQIQRLVLSTVVIFLACFLPYHVLLLVRSVWEASCDFAKGVFNAYHFSLLLTSFNCVADPVLYCFVSETTHRDLARLRGACLAFLTCSRTGRAREAYPLGAPEASGKSGAQGEEPELLTKLHPAFQTPNSPGSGGFPTGRLA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
73
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Proton-sensing receptor involved in pH homeostasis. May represents an osteoblastic pH sensor regulating cell-mediated responses to acidosis in bone. Mediates its action by association with G proteins that stimulates inositol phosphate (IP) production or Ca(2+) mobilization. The receptor is almost silent at pH 7.8 but fully activated at pH 6.8. Also functions as a metastasis suppressor gene in prostate cancer (By similarity).

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Found at low level in a wide range of tissues, but significantly expressed in lung, kidney, bone and nervous system.

タンパク質ファミリー:

Belongs to the G-protein coupled receptor 1 family.

参考文献

1). The impact of high-intensity interval training on cerebrovascular function in the APP/PS1 mice. Frontiers in aging, 2025 (PubMed: 41164084) [IF=3.3]

Application: WB    Species: Mouse    Sample:

FIGURE 6 The effect of HIIT on the cerebrovascular regulation of APP/PS1 mice. (A) the Western blot image of eNOS, GPR68, and ET-1. (B) quantification of eNOS in each group. (C) quantification of GPR81 in each group. (D) quantification of ET-1 in each group. (E) the mRNA expression of Nos1. (F) the mRNA expression of Gpr68. (G) the mRNA expression of Edn1. β-ACTIN and Gapdh are internal controls for quantification. Data are displayed as mean ± SE (n = 7). *: p < 0.01 APP/PS1 group versus Control group; #: p < 0.01 APP/PS1 + HIIT group versus APP/PS1 group.

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