ANKRD1 Antibody - #AF0677

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製品: ANKRD1 Antibody
カタログ: AF0677
タンパク質の説明: Rabbit polyclonal antibody to ANKRD1
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 36kDa; 36kD(Calculated).
ユニプロット: Q15327
RRID: AB_2834258

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WB Handbook
IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
ANKRD1 Antibody detects endogenous levels of total ANKRD1.
RRID:
AB_2834258
引用形式: Affinity Biosciences Cat# AF0677, RRID:AB_2834258.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ALRP; ANKR1_HUMAN; ANKRD 1; ANKRD1; Ankyrin repeat domain 1 (cardiac muscle); Ankyrin repeat domain containing protein 1; Ankyrin repeat domain-containing protein 1; bA320F15.2; C 193; C193; Cardiac ankyrin repeat protein; CARP; CVARP; Cytokine inducible nuclear protein; Cytokine-inducible gene C-193 protein; Cytokine-inducible nuclear protein; HA1A2; Liver ankyrin repeat domain 1; MCARP; OTTHUMP00000020084;

免疫原

免疫原:

A synthesized peptide derived from human ANKRD1, corresponding to a region within the internal amino acids.

Uniprot:

Q15327(ANKR1_HUMAN) >>Visit HPA database.

遺伝子(ID):
ANKRD1(27063)
発現特異性:
Q15327 ANKR1_HUMAN:

Mainly expressed in activated vascular endothelial cells. To a lower extent, also expressed in hepatoma cells.

タンパク質の説明:
The protein encoded by this gene is localized to the nucleus of endothelial cells and is induced by IL-1 and TNF-alpha stimulation. Studies in rat cardiomyocytes suggest that this gene functions as a transcription factor.
タンパク質配列:
MMVLKVEELVTGKKNGNGEAGEFLPEDFRDGEYEAAVTLEKQEDLKTLLAHPVTLGEQQWKSEKQREAELKKKKLEQRSKLENLEDLEIIIQLKKRKKYRKTKVPVVKEPEPEIITEPVDVPTFLKAALENKLPVVEKFLSDKNNPDVCDEYKRTALHRACLEGHLAIVEKLMEAGAQIEFRDMLESTAIHWASRGGNLDVLKLLLNKGAKISARDKLLSTALHVAVRTGHYECAEHLIACEADLNAKDREGDTPLHDAVRLNRYKMIRLLIMYGADLNIKNCAGKTPMDLVLHWQNGTKAIFDSLRENSYKTSRIATF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

May play an important role in endothelial cell activation. May act as a nuclear transcription factor that negatively regulates the expression of cardiac genes. Induction seems to be correlated with apoptotic cell death in hepatoma cells.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Mainly expressed in activated vascular endothelial cells. To a lower extent, also expressed in hepatoma cells.

参考文献

1). LINC00525 drives aggressive phenotypes in bladder cancer via YAP stabilization-mediated transcriptional activation. Cancer cell international, 2025 (PubMed: 40528165) [IF=5.8]

Application: WB    Species: Mouse    Sample:

Fig. 6 LINC00525 regulates bladder cancer tumor growth through YAP. A and B. The volume and growth of tumors in the following groups (sh Control, sh-00525, sh-00525 + GA-017). C. Immunohistochemical staining shows the expression of KI67, YAP and pYAPS127 in mice tumors among sh Control, sh-00525, sh-00525 + GA-017 groups. Scale bar: 100 μm. D. Relative protein expression of YAP, pYAPS127, downstream targets genes (such as CYR61, CTGF and ANKRD1) and LINC00525 in mice tumors among sh Control, sh-00525, sh-00525 + GA-017 groups. E-H. The volume and growth of tumors in the following groups (Control, LINC00525, LINC00525 + Cytochalasin D) and analyzed as in C and D
2). Storax Attenuates Cardiac Fibrosis following Acute Myocardial Infarction in Rats via Suppression of AT1R–Ankrd1–P53 Signaling Pathway. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022 (PubMed: 36361958) [IF=5.6]

Application: WB    Species: Rat    Sample: cardiac tissue

Figure 8 Storax inhibits the AT1R–Ankrd1–P53 pathway in AMI rats against cardiomyocyte apoptosis. (A) WB bands showing the protein expression levels of AT1R, Ankrd1, P-p53 (ser15), P53 and Mdm2 in cardiac tissue. (B–E) Relative protein for AT1R, Ankrd1, P-p53 (ser15) and Mdm2 were quantified by densitometry based on immunoblot images. Results are presented as mean ± SD (n = 3). One-way ANOVA follow by Bonferroni’s post hoc test: # p < 0.05, ## p < 0.01 vs. vehicle group.

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